Restriction map for
pFN21AB9739
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccI AccII AclI AflII AflIII
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
BalI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BsaAI
BsaBI BseRI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KasI KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 888 GACGTC GACGT^C
Acc65I 399 GGTACC G^GTACC
AccIII 461 TCCGGA T^CCGGA
AcyI 888 GACGTC G[GA]^CG[CT]C
AfaI 400 GTAC GT^AC
AgeI 1346 ACCGGT A^CCGGT
AocI 1172 CCTCAGG CC^T[ACGT]AGG
ApaI 1059 GGGCCC GGGCC^C
Asp718I 399 GGTACC G^GTACC
AspI 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 629 CTCGGG C^[CT]CG[GA]G
AviII 99 TGCGCA TGC^GCA
AvrII 964 CCTAGG C^CTAGG
BamHI 1312 GGATCC G^GATCC
BcgI 1178 GCATGTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 964 CCTAGG C^CTAGG
BlpI 454 GCTGAGC GC^T[ACGT]AGC
BpmI 48 CTCCAG CTGGAG(16/14)
Bpu1102I 454 GCTGAGC GC^T[ACGT]AGC
BsaHI 888 GACGTC G[GA]^CG[CT]C
BseAI 461 TCCGGA T^CCGGA
BsgI 773 CTGCAC GTGCAG(16/14)
BsoBI 629 CTCGGG C^[CT]CG[GA]G
BspEI 461 TCCGGA T^CCGGA
BstXI 1425 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1172 CCTCAGG CC^T[ACGT]AGG
CelII 454 GCTGAGC GC^T[ACGT]AGC
DraII 1059 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 888 GACGTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1172 CCTCAGG CC^T[ACGT]AGG
EcoNI 390 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1059 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1479 GAATTC G^AATTC
FspI 99 TGCGCA TGC^GCA
HgaI 1441 GACGC GACGC(5/10)
Hin1I 888 GACGTC G[GA]^CG[CT]C
HincII 1438 GTTGAC GT[CT]^[GA]AC
HindII 1438 GTTGAC GT[CT]^[GA]AC
HindIII 655 AAGCTT A^AGCTT
KpnI 399 GGTACC GGTAC^C
MroI 461 TCCGGA T^CCGGA
NaeI 29 GCCGGC GCC^GGC
NgoMIV 29 GCCGGC G^CCGGC
NotI 237 GCGGCCGC GC^GGCCGC
NspI 1178 GCATGT [GA]CATG^[CT]
PflFI 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1346 ACCGGT A^CCGGT
PvuII 218 CAGCTG CAG^CTG
RsaI 400 GTAC GT^AC
SacI 457 GAGCTC GAGCT^C
TaqI 1387 TCGA T^CGA
TfiI 1501 GAATC G^A[AT]TC
Tsp45I 1168 GTCAC ^GT[GC]AC
Tth111I 800 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1387 TCGA T^CGA
XcmI 1248 CCAAAGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1413 AAATTT [GA]^AATT[CT]
1479 GAATTC
ApoI 1413 AAATTT [GA]^AATT[CT]
1479 GAATTC
BanI 399 GGTACC G^G[CT][GA]CC
1332 GGTGCC
BsaI 1343 GAGACC GGTCTC(1/5)
1349 GGTCTC
BsaWI 461 TCCGGA [AT]^CCGG[AT]
1346 ACCGGT
BsiEI 238 CGGCCG CG[GA][CT]^CG
287 CGGCCG
BsmAI 1343 GAGAC GTCTC(1/5)
1350 GTCTC
BspMI 1220 ACCTGC ACCTGC(4/8)
1042 GCAGGT
BsrFI 29 GCCGGC [GA]^CCGG[CT]
1346 ACCGGT
BstYI 15 AGATCC [GA]^GATC[CT]
1312 GGATCC
Cfr10I 29 GCCGGC [GA]^CCGG[CT]
1346 ACCGGT
EaeI 238 CGGCCG [CT]^GGCC[GA]
287 CGGCCG
EagI 238 CGGCCG C^GGCCG
287 CGGCCG
EarI 1242 CTCTTC CTCTTC(1/4)
1032 GAAGAG
EclXI 238 CGGCCG C^GGCCG
287 CGGCCG
Eco52I 238 CGGCCG C^GGCCG
287 CGGCCG
HaeII 32 GGCGCT [GA]GCGC^[CT]
347 GGCGCT
Ksp632I 1242 CTCTTC CTCTTC(1/4)
1032 GAAGAG
MaeII 80 ACGT A^CGT
889 ACGT
MflI 15 AGATCC [GA]^GATC[CT]
1312 GGATCC
MslI 258 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
954 CACCGATGTG
MspA1I 218 CAGCTG C[AC]G^C[GT]G
916 CCGCTG
NcoI 260 CCATGG C^CATGG
1328 CCATGG
PleI 664 GACTC GAGTC(4/5)
804 GAGTC
PstI 58 CTGCAG CTGCA^G
221 CTGCAG
SapI 1032 GAAGAGC GCTCTTC(1/4)
1241 GCTCTTC
SfcI 58 CTGCAG C^T[GA][CT]AG
221 CTGCAG
XhoII 15 AGATCC [GA]^GATC[CT]
1312 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI Tru9I
TseI Tsp509I TspRI