Restriction map for
pFN21AE5056
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BclI BfrI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII Hin1I HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 455 GTCGAC GT^[AC][GT]AC
AccIII 167 TCCGGA T^CCGGA
AlwNI 375 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 257 CTCGGG C^[CT]CG[GA]G
AviII 527 TGCGCA TGC^GCA
BalI 26 TGGCCA TGG^CCA
BcgI 249 GCAGGCATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 262 GGATAC GTATCC(6/5)
BcnI 188 CCGGG CC^[GC]GG
BfaI 268 CTAG C^TAG
BglI 28 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 167 TCCGGA [AT]^CCGG[AT]
BseAI 167 TCCGGA T^CCGGA
BsoBI 257 CTCGGG C^[CT]CG[GA]G
BspEI 167 TCCGGA T^CCGGA
BspHI 533 TCATGA T^CATGA
BspMI 431 ACCTGC ACCTGC(4/8)
BsrI 162 CCAGT ACTGG(1/-1)
BstAPI 385 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 550 TTCGAA TT^CGAA
CfoI 528 GCGC GCG^C
DraI 154 TTTAAA TTT^AAA
EaeI 26 TGGCCA [CT]^GGCC[GA]
EcoT14I 596 CCAAGG C^C[AT][AT]GG
FspI 527 TGCGCA TGC^GCA
HgaI 435 GCGTC GACGC(5/10)
HhaI 528 GCGC GCG^C
HinP1I 528 GCGC G^CGC
HincII 455 GTCGAC GT[CT]^[GA]AC
HindII 455 GTCGAC GT[CT]^[GA]AC
HindIII 302 AAGCTT A^AGCTT
MaeI 268 CTAG C^TAG
MluNI 26 TGGCCA TGG^CCA
MroI 167 TCCGGA T^CCGGA
MscI 26 TGGCCA TGG^CCA
MseI 155 TTAA T^TAA
MslI 444 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 188 CCGGG CC^[GC]GG
NspI 279 GCATGC [GA]CATG^[CT]
NspV 550 TTCGAA TT^CGAA
PleI 458 GACTC GAGTC(4/5)
RcaI 533 TCATGA T^CATGA
SalI 455 GTCGAC G^TCGAC
SexAI 358 ACCTGGT A^CC[AT]GGT
SfuI 550 TTCGAA TT^CGAA
SphI 279 GCATGC GCATG^C
StuI 599 AGGCCT AGG^CCT
StyI 596 CCAAGG C^C[AT][AT]GG
TfiI 592 GAATC G^A[AT]TC
Tru9I 155 TTAA T^TAA
TspRI 30 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 157 AAATTC [GA]^AATT[CT]
567 GAATTT
AfaI 180 GTAC GT^AC
348 GTAC
AlwI 483 GATCC GGATC(4/5)
57 GGATC
ApoI 157 AAATTC [GA]^AATT[CT]
567 GAATTT
BpmI 372 CTCCAG CTGGAG(16/14)
61 CTGGAG
BseRI 472 GAGGAG GAGGAG(10/8)
646 GAGGAG
BsgI 247 GTGCAG GTGCAG(16/14)
383 GTGCAG
BstYI 57 GGATCT [GA]^GATC[CT]
482 AGATCC
Cfr13I 3 GGCCC G^G[ACGT]CC
240 GGGCC
DpnII 58 GATC ^GATC
483 GATC
HinfI 458 GACTC G^A[ACGT]TC
592 GAATC
HphI 589 GGTGA GGTGA(8/7)
356 TCACC
MaeIII 124 GTCAC ^GT[ACGT]AC
355 GTCAC
MboI 58 GATC ^GATC
483 GATC
MflI 57 GGATCT [GA]^GATC[CT]
482 AGATCC
NdeII 58 GATC ^GATC
483 GATC
NlaIV 239 GGGGCC GG[ACGT]^[ACGT]CC
474 GGAGCC
RsaI 180 GTAC GT^AC
348 GTAC
Sau3AI 58 GATC ^GATC
483 GATC
Sau96I 3 GGCCC G^G[ACGT]CC
240 GGGCC
SfaNI 253 GCATC GCATC(5/9)
530 GCATC
Tsp45I 124 GTCAC ^GT[GC]AC
355 GTCAC
Tsp509I 158 AATT ^AATT
568 AATT
XhoII 57 GGATCT [GA]^GATC[CT]
482 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
DdeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NlaIII ScrFI TaqI TseI TthHB8I