Restriction map for
pFN21AE3152
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AlwNI AocI Aor51HI AscI
AseI AsnI Asp700I AspI AviII
AvrII BbrPI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsmBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MfeI MluI MunI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 310 GGTACC G^GTACC
AccIII 926 TCCGGA T^CCGGA
AcsI 709 AAATTT [GA]^AATT[CT]
AhdI 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1015 GTGCAC G^TGCAC
ApaLI 1015 GTGCAC G^TGCAC
ApoI 709 AAATTT [GA]^AATT[CT]
Asp718I 310 GGTACC G^GTACC
AspEI 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 113 TGGCCA TGG^CCA
BamHI 408 GGATCC G^GATCC
BbsI 262 GAAGAC GAAGAC(2/6)
BbvCI 510 GCTGAGG CCTCAGC(-5/-2)
BcgI 615 CGACTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 262 GAAGAC GAAGAC(2/6)
BsaBI 591 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 926 TCCGGA [AT]^CCGG[AT]
BseAI 926 TCCGGA T^CCGGA
BsgI 703 GTGCAG GTGCAG(16/14)
BsiWI 240 CGTACG C^GTACG
BsmI 813 GAATGC GAATGC(1/-1)
Bsp1407I 524 TGTACA T^GTACA
BspEI 926 TCCGGA T^CCGGA
BsrGI 524 TGTACA T^GTACA
BstF5I 621 GGATG GGATG(2/0)
BstYI 408 GGATCC [GA]^GATC[CT]
EagI 183 CGGCCG C^GGCCG
Eam1105I 237 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 183 CGGCCG C^GGCCG
Eco52I 183 CGGCCG C^GGCCG
FokI 621 GGATG GGATG(9/13)
HincII 100 GTTGAC GT[CT]^[GA]AC
HindII 100 GTTGAC GT[CT]^[GA]AC
KpnI 310 GGTACC GGTAC^C
KspI 180 CCGCGG CCGC^GG
MaeIII 245 GTCAC ^GT[ACGT]AC
MamI 591 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 408 GGATCC [GA]^GATC[CT]
MluNI 113 TGGCCA TGG^CCA
MroI 926 TCCGGA T^CCGGA
MscI 113 TGGCCA TGG^CCA
MslI 105 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 627 CCATGG C^CATGG
NheI 855 GCTAGC G^CTAGC
PpuMI 740 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 657 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 961 GAGCTC GAGCT^C
SacII 180 CCGCGG CCGC^GG
SfcI 651 CTACAG C^T[GA][CT]AG
SgrAI 117 CACCGGCG C[GA]^CCGG[CT]G
SmlI 667 CTCAAG C^T[CT][GA]AG
SspBI 524 TGTACA T^GTACA
Tsp45I 245 GTCAC ^GT[GC]AC
XhoII 408 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 194 GGGCCC GGGCC^C
1031 GGGCCC
AspHI 961 GAGCTC G[AT]GC[AT]^C
1015 GTGCAC
BsaI 235 GAGACC GGTCTC(1/5)
361 GAGACC
BseRI 1072 CTCCTC GAGGAG(10/8)
513 GAGGAG
BsiHKAI 961 GAGCTC G[AT]GC[AT]^C
1015 GTGCAC
BssHII 436 GCGCGC G^CGCGC
438 GCGCGC
EcoNI 202 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
480 CCTGTTTGAGG
EcoT14I 163 CCTTGG C^C[AT][AT]GG
627 CCATGG
HgaI 225 GACGC GACGC(5/10)
490 GACGC
MaeII 110 ACGT A^CGT
243 ACGT
MseI 374 TTAA T^TAA
896 TTAA
NaeI 185 GCCGGC GCC^GGC
971 GCCGGC
NgoMIV 185 GCCGGC G^CCGGC
971 GCCGGC
PflMI 730 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCAGCTAGTGG
ScaI 723 AGTACT AGT^ACT
887 AGTACT
StuI 477 AGGCCT AGG^CCT
671 AGGCCT
StyI 163 CCTTGG C^C[AT][AT]GG
627 CCATGG
TaqI 23 TCGA T^CGA
614 TCGA
TfiI 596 GAATC G^A[AT]TC
878 GATTC
Tru9I 374 TTAA T^TAA
896 TTAA
Tsp509I 633 AATT ^AATT
710 AATT
TthHB8I 23 TCGA T^CGA
614 TCGA
Van91I 730 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCAGCTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI SmaI TseI TspRI
XmaCI XmaI