Restriction map for
pFN21AE1709
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcyI 466 GGCGCC G[GA]^CG[CT]C
AvaI 514 CCCGAG C^[CT]CG[GA]G
BanI 466 GGCGCC G^G[CT][GA]CC
BbeI 466 GGCGCC GGCGC^C
BfaI 148 CTAG C^TAG
BpmI 166 CTGGAG CTGGAG(16/14)
BsaHI 466 GGCGCC G[GA]^CG[CT]C
BsaI 531 GAGACC GGTCTC(1/5)
BseRI 13 GAGGAG GAGGAG(10/8)
BsmFI 127 GTCCC GGGAC(10/14)
BsoBI 514 CCCGAG C^[CT]CG[GA]G
Bsp1407I 428 TGTACA T^GTACA
BsrDI 53 GCAATG GCAATG(2/0)
BsrFI 521 ACCGGC [GA]^CCGG[CT]
BsrGI 428 TGTACA T^GTACA
BssSI 329 CTCGTG CACGAG(-5/-1)
BstXI 413 CCAAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 572 AGATCC [GA]^GATC[CT]
CfoI 467 GCGC GCG^C
Cfr10I 521 ACCGGC [GA]^CCGG[CT]
DdeI 9 CTCAG C^T[ACGT]AG
DraII 125 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 499 CTCTTC CTCTTC(1/4)
EcoO109I 125 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 58 GAATTC G^AATTC
EcoT14I 392 CCATGG C^C[AT][AT]GG
FauI 254 CCCGC CCCGC(4/6)
HaeII 466 GGCGCC [GA]GCGC^[CT]
HapII 522 CCGG C^CGG
HhaI 467 GCGC GCG^C
Hin1I 466 GGCGCC G[GA]^CG[CT]C
HinP1I 467 GCGC G^CGC
HpaII 522 CCGG C^CGG
KasI 466 GGCGCC G^GCGCC
Ksp632I 499 CTCTTC CTCTTC(1/4)
MaeI 148 CTAG C^TAG
MflI 572 AGATCC [GA]^GATC[CT]
MseI 101 TTAA T^TAA
MspA1I 592 CAGCTG C[AC]G^C[GT]G
MspI 522 CCGG C^CGG
MwoI 410 GCCCCAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 466 GGCGCC GG^CGCC
NcoI 392 CCATGG C^CATGG
NspI 494 ACATGC [GA]CATG^[CT]
PleI 113 GACTC GAGTC(4/5)
PpuMI 125 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 595 CTGCAG CTGCA^G
PvuII 592 CAGCTG CAG^CTG
SapI 498 GCTCTTC GCTCTTC(1/4)
SfcI 595 CTGCAG C^T[GA][CT]AG
SfoI 466 GGCGCC GGC^GCC
SspBI 428 TGTACA T^GTACA
StyI 392 CCATGG C^C[AT][AT]GG
TaqI 138 TCGA T^CGA
TfiI 639 GATTC G^A[AT]TC
Tru9I 101 TTAA T^TAA
Tsp45I 206 GTGAC ^GT[GC]AC
TspRI 480 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 138 TCGA T^CGA
XbaI 147 TCTAGA T^CTAGA
XcmI 453 CCATCACACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 572 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 255 CCGC CCGC(-3/-1)
608 CCGC
AcsI 58 GAATTC [GA]^AATT[CT]
97 AAATTT
AlwI 246 GATCC GGATC(4/5)
573 GATCC
ApoI 58 GAATTC [GA]^AATT[CT]
97 AAATTT
AvaII 126 GGTCC G^G[AT]CC
162 GGACC
BanII 43 GGGCTC G[GA]GC[CT]^C
624 GAGCCC
BbsI 79 GAAGAC GAAGAC(2/6)
187 GAAGAC
BpuAI 79 GAAGAC GAAGAC(2/6)
187 GAAGAC
BsmAI 311 GAGAC GTCTC(1/5)
531 GAGAC
BspMI 534 ACCTGC ACCTGC(4/8)
123 GCAGGT
HinfI 113 GACTC G^A[ACGT]TC
639 GATTC
HphI 385 GGTGA GGTGA(8/7)
396 GGTGA
MaeIII 206 GTGAC ^GT[ACGT]AC
320 GTTAC
MslI 420 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
457 CACACGGATG
PflMI 285 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
576 CCATCCGATGG
Tsp509I 59 AATT ^AATT
98 AATT
Van91I 285 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
576 CCATCCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
ItaI MboI MboII MnlI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI