Restriction map for
pFN21AE6263
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 141 GTAC GT^AC
AlwI 299 GGATC GGATC(4/5)
Aor51HI 425 AGCGCT AGC^GCT
Asp700I 108 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 123 GGTCC G^G[AT]CC
AvrII 529 CCTAGG C^CTAGG
BcnI 290 CCCGG CC^[GC]GG
BfaI 530 CTAG C^TAG
BglII 327 AGATCT A^GATCT
BlnI 529 CCTAGG C^CTAGG
BsmAI 406 GAGAC GTCTC(1/5)
BsmFI 486 GTCCC GGGAC(10/14)
BstEII 418 GGTTACC G^GT[ACGT]ACC
BstPI 418 GGTTACC G^GT[ACGT]ACC
Cac8I 223 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 426 GCGC GCG^C
DrdI 199 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 425 AGCGCT AGC^GCT
EcoO65I 418 GGTTACC G^GT[ACGT]ACC
EcoRI 478 GAATTC G^AATTC
HaeII 425 AGCGCT [GA]GCGC^[CT]
HapII 291 CCGG C^CGG
HhaI 426 GCGC GCG^C
HinP1I 426 GCGC G^CGC
HincII 80 GTCAAC GT[CT]^[GA]AC
HindII 80 GTCAAC GT[CT]^[GA]AC
HpaII 291 CCGG C^CGG
HphI 448 TCACC GGTGA(8/7)
MaeI 530 CTAG C^TAG
MaeIII 419 GTTAC ^GT[ACGT]AC
MseI 50 TTAA T^TAA
MspI 291 CCGG C^CGG
NciI 290 CCCGG CC^[GC]GG
RsaI 141 GTAC GT^AC
SacI 364 GAGCTC GAGCT^C
SfaNI 237 GATGC GCATC(5/9)
StuI 542 AGGCCT AGG^CCT
Tru9I 50 TTAA T^TAA
XcmI 167 CCAAGGAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 108 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 264 CCGC CCGC(-3/-1)
345 CCGC
AcsI 11 AAATTT [GA]^AATT[CT]
478 GAATTC
ApoI 11 AAATTT [GA]^AATT[CT]
478 GAATTC
BanII 277 GGGCTC G[GA]GC[CT]^C
364 GAGCTC
BbsI 357 GAAGAC GAAGAC(2/6)
208 GTCTTC
BpuAI 357 GAAGAC GAAGAC(2/6)
208 GTCTTC
BseRI 433 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsiYI 9 CCAAATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
290 CCCGGAAATGG
BslI 9 CCAAATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
290 CCCGGAAATGG
BsrI 63 CCAGT ACTGG(1/-1)
135 CCAGT
BstYI 299 GGATCT [GA]^GATC[CT]
327 AGATCT
Cfr13I 123 GGTCC G^G[ACGT]CC
132 GGGCC
DpnII 300 GATC ^GATC
328 GATC
EcoT14I 167 CCAAGG C^C[AT][AT]GG
529 CCTAGG
MboI 300 GATC ^GATC
328 GATC
MboII 357 GAAGA GAAGA(8/7)
209 TCTTC
MflI 299 GGATCT [GA]^GATC[CT]
327 AGATCT
MslI 247 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CATCCGCATG
NdeII 300 GATC ^GATC
328 GATC
PleI 505 GACTC GAGTC(4/5)
525 GACTC
Sau3AI 300 GATC ^GATC
328 GATC
Sau96I 123 GGTCC G^G[ACGT]CC
132 GGGCC
StyI 167 CCAAGG C^C[AT][AT]GG
529 CCTAGG
TfiI 392 GAATC G^A[AT]TC
534 GAATC
Tsp509I 12 AATT ^AATT
479 AATT
TspRI 64 CAGTG CA[GC]TG[ACGT][ACGT]^
136 CAGTG
XhoII 299 GGATCT [GA]^GATC[CT]
327 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsaJI
BsiHKAI Bsp1286I BssKI BstF5I BstNI
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MnlI MvaI MwoI
NlaIII ScrFI TseI