Restriction map for
pFN21AE6501
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstF5I BstUI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MslI MspA1I MunI MvnI NarI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 402 GTAC GT^AC
AluI 17 AGCT AG^CT
ApaI 135 GGGCCC GGGCC^C
AvaI 392 CCCGGG C^[CT]CG[GA]G
BalI 160 TGGCCA TGG^CCA
BanI 168 GGCACC G^G[CT][GA]CC
BanII 135 GGGCCC G[GA]GC[CT]^C
BbvI 65 GCAGC GCAGC(8/12)
BsaI 221 GAGACC GGTCTC(1/5)
BsgI 43 CTGCAC GTGCAG(16/14)
BsmAI 221 GAGAC GTCTC(1/5)
BsoBI 392 CCCGGG C^[CT]CG[GA]G
BspMI 224 ACCTGC ACCTGC(4/8)
BsrDI 125 CATTGC GCAATG(2/0)
BsrFI 303 GCCGGC [GA]^CCGG[CT]
BstEII 151 GGTGACC G^GT[ACGT]ACC
BstPI 151 GGTGACC G^GT[ACGT]ACC
Cfr10I 303 GCCGGC [GA]^CCGG[CT]
EarI 397 GAAGAG CTCTTC(1/4)
EcoNI 157 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 151 GGTGACC G^GT[ACGT]ACC
Fnu4HI 65 GCAGC GC^[ACGT]GC
HinfI 239 GAGTC G^A[ACGT]TC
ItaI 65 GCAGC GC^[ACGT]GC
Ksp632I 397 GAAGAG CTCTTC(1/4)
MaeIII 152 GTGAC ^GT[ACGT]AC
MluNI 160 TGGCCA TGG^CCA
MscI 160 TGGCCA TGG^CCA
NaeI 303 GCCGGC GCC^GGC
NgoMIV 303 GCCGGC G^CCGGC
PleI 239 GAGTC GAGTC(4/5)
PpuMI 309 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 402 GTAC GT^AC
SfaNI 56 GCATC GCATC(5/9)
SmaI 392 CCCGGG CCC^GGG
TseI 65 GCAGC G^C[AT]GC
Tsp45I 152 GTGAC ^GT[GC]AC
XcmI 322 CCAGAAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 392 CCCGGG C^CCGGG
XmaI 392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BamHI 26 GGATCC G^GATCC
267 GGATCC
BfaI 260 CTAG C^TAG
382 CTAG
BmyI 135 GGGCCC G[AGT]GC[ACT]^C
167 GGGCAC
BpmI 216 CTGGAG CTGGAG(16/14)
236 CTGGAG
BsmFI 145 GTCCC GGGAC(10/14)
212 GTCCC
Bsp1286I 135 GGGCCC G[AGT]GC[ACT]^C
167 GGGCAC
EaeI 160 TGGCCA [CT]^GGCC[GA]
301 TGGCCG
EcoT14I 157 CCTTGG C^C[AT][AT]GG
298 CCTTGG
FauI 91 CCCGC CCCGC(4/6)
200 CCCGC
HphI 151 GGTGA GGTGA(8/7)
84 TCACC
MaeI 260 CTAG C^TAG
382 CTAG
MboII 184 GAAGA GAAGA(8/7)
397 GAAGA
MwoI 56 GCATCTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
65 GCAGCGAGGGC
StyI 157 CCTTGG C^C[AT][AT]GG
298 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AlwI AocI AvaII BcnI
BsaJI BseRI BsiYI BslI BsrI
BssKI BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII Eco81I
EcoO109I EcoRII HaeIII HapII HpaII
MboI MflI MnlI MspI MvaI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI XhoII