Restriction map for
pFN21AE2319
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MflI MluI MroI
MseI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 316 GTAC GT^AC
AocI 63 CCTGAGG CC^T[ACGT]AGG
ApaI 764 GGGCCC GGGCC^C
BalI 399 TGGCCA TGG^CCA
BanII 764 GGGCCC G[GA]GC[CT]^C
BciVI 298 GGATAC GTATCC(6/5)
BmyI 764 GGGCCC G[AGT]GC[ACT]^C
BsaI 520 GAGACC GGTCTC(1/5)
BsiEI 256 CGACCG CG[GA][CT]^CG
Bsp1286I 764 GGGCCC G[AGT]GC[ACT]^C
BssSI 620 CTCGTG CACGAG(-5/-1)
BstAPI 864 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 63 CCTGAGG CC^T[ACGT]AGG
EaeI 399 TGGCCA [CT]^GGCC[GA]
EarI 415 CTCTTC CTCTTC(1/4)
Eco81I 63 CCTGAGG CC^T[ACGT]AGG
EcoNI 596 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 22 GACGC GACGC(5/10)
Ksp632I 415 CTCTTC CTCTTC(1/4)
MaeII 954 ACGT A^CGT
MboII 416 TCTTC GAAGA(8/7)
MfeI 464 CAATTG C^AATTG
MluNI 399 TGGCCA TGG^CCA
MscI 399 TGGCCA TGG^CCA
MspA1I 647 CCGCTG C[AC]G^C[GT]G
MunI 464 CAATTG C^AATTG
NaeI 99 GCCGGC GCC^GGC
NgoMIV 99 GCCGGC G^CCGGC
PpuMI 837 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 522 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 629 CTGCAG CTGCA^G
RsaI 316 GTAC GT^AC
SpeI 193 ACTAGT A^CTAGT
TfiI 174 GAATC G^A[AT]TC
Tsp45I 713 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 24 CGCG CG^CG
775 CGCG
AcsI 493 AAATTC [GA]^AATT[CT]
721 GAATTT
AlwI 637 GATCC GGATC(4/5)
41 GGATC
ApoI 493 AAATTC [GA]^AATT[CT]
721 GAATTT
AvaII 54 GGACC G^G[AT]CC
838 GGTCC
BcnI 33 CCCGG CC^[GC]GG
85 CCCGG
BfaI 194 CTAG C^TAG
710 CTAG
BglI 253 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
744 GCCTCCTTGGC
BseRI 705 CTCCTC GAGGAG(10/8)
985 GAGGAG
BsmAI 520 GAGAC GTCTC(1/5)
689 GAGAC
BsmFI 529 GTCCC GGGAC(10/14)
839 GTCCC
BsrFI 99 GCCGGC [GA]^CCGG[CT]
258 ACCGGC
BstUI 24 CGCG CG^CG
775 CGCG
BstXI 396 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAGCAGCCTGG
Cfr10I 99 GCCGGC [GA]^CCGG[CT]
258 ACCGGC
DsaI 396 CCATGG C^C[GA][CT]GG
402 CCATGG
HphI 537 GGTGA GGTGA(8/7)
826 TCACC
MaeI 194 CTAG C^TAG
710 CTAG
MaeIII 215 GTAAC ^GT[ACGT]AC
713 GTGAC
MslI 397 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
616 CACTCTCGTG
MvnI 24 CGCG CG^CG
775 CGCG
NciI 33 CCCGG CC^[GC]GG
85 CCCGG
NcoI 396 CCATGG C^CATGG
402 CCATGG
PleI 382 GAGTC GAGTC(4/5)
990 GAGTC
SfcI 115 CTACAG C^T[GA][CT]AG
629 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwNI BbvI BpmI
BsaJI BsiYI BslI BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp509I TthHB8I