Restriction map for
pFN21AE5386
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MflI
MluI MroI MspA1I MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 25 GAATTT [GA]^AATT[CT]
AlwI 148 GATCC GGATC(4/5)
ApaI 618 GGGCCC GGGCC^C
ApoI 25 GAATTT [GA]^AATT[CT]
BalI 425 TGGCCA TGG^CCA
BanII 618 GGGCCC G[GA]GC[CT]^C
BbvCI 406 CCTCAGC CCTCAGC(-5/-2)
BciVI 512 GTATCC GTATCC(6/5)
BclI 908 TGATCA T^GATCA
BfaI 152 CTAG C^TAG
BglI 340 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 618 GGGCCC G[AGT]GC[ACT]^C
BsaWI 809 ACCGGA [AT]^CCGG[AT]
BseRI 31 CTCCTC GAGGAG(10/8)
BsgI 466 CTGCAC GTGCAG(16/14)
BsmFI 604 GGGAC GGGAC(10/14)
Bsp1286I 618 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 827 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrDI 542 CATTGC GCAATG(2/0)
BsrGI 827 TGTACA T^GTACA
BsrI 112 ACTGG ACTGG(1/-1)
DsaI 614 CCATGG C^C[GA][CT]GG
EaeI 425 TGGCCA [CT]^GGCC[GA]
EcoRV 202 GATATC GAT^ATC
EcoT22I 282 ATGCAT ATGCA^T
FbaI 908 TGATCA T^GATCA
HapII 810 CCGG C^CGG
HpaII 810 CCGG C^CGG
MaeI 152 CTAG C^TAG
MfeI 608 CAATTG C^AATTG
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MslI 102 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 810 CCGG C^CGG
MunI 608 CAATTG C^AATTG
NcoI 614 CCATGG C^CATGG
NsiI 282 ATGCAT ATGCA^T
NspI 692 GCATGC [GA]CATG^[CT]
RcaI 398 TCATGA T^CATGA
ScaI 592 AGTACT AGT^ACT
SfaNI 281 GATGC GCATC(5/9)
SphI 692 GCATGC GCATG^C
SspBI 827 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 801 GGACC G^G[AT]CC
807 GGACC
BsaBI 135 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
423 GATGGCCATC
BstF5I 778 CATCC GGATG(2/0)
280 GGATG
DraII 618 GGGCCCC [GA]G^G[ACGT]CC[CT]
892 GGGGCCT
EarI 73 CTCTTC CTCTTC(1/4)
667 CTCTTC
EcoO109I 618 GGGCCCC [GA]G^G[ACGT]CC[CT]
892 GGGGCCT
EcoT14I 614 CCATGG C^C[AT][AT]GG
896 CCTTGG
FokI 778 CATCC GGATG(9/13)
280 GGATG
HinfI 155 GACTC G^A[ACGT]TC
325 GAGTC
Ksp632I 73 CTCTTC CTCTTC(1/4)
667 CTCTTC
MamI 135 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
423 GATGGCCATC
MseI 536 TTAA T^TAA
575 TTAA
MwoI 228 GCCAAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
340 GCCGTGATGGC
PflMI 306 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCACTTCATGG
PleI 155 GACTC GAGTC(4/5)
325 GAGTC
PstI 61 CTGCAG CTGCA^G
238 CTGCAG
SfcI 61 CTGCAG C^T[GA][CT]AG
238 CTGCAG
StyI 614 CCATGG C^C[AT][AT]GG
896 CCTTGG
Tru9I 536 TTAA T^TAA
575 TTAA
Van91I 306 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCACTTCATGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HphI ItaI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
Tsp45I Tsp509I TspRI