Restriction map for
pFN21AE5580
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 1004 CCGC CCGC(-3/-1)
AcsI 1063 AAATTT [GA]^AATT[CT]
AflII 490 CTTAAG C^TTAAG
AlwI 522 GGATC GGATC(4/5)
ApoI 1063 AAATTT [GA]^AATT[CT]
Asp700I 331 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 207 GGACC G^G[AT]CC
BcnI 807 CCGGG CC^[GC]GG
BfaI 67 CTAG C^TAG
BfrI 490 CTTAAG C^TTAAG
BspMI 776 ACCTGC ACCTGC(4/8)
BsrDI 840 CATTGC GCAATG(2/0)
DraI 901 TTTAAA TTT^AAA
DraIII 500 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 61 CCATGG C^C[GA][CT]GG
EaeI 344 TGGCCG [CT]^GGCC[GA]
EarI 826 CTCTTC CTCTTC(1/4)
EcoNI 899 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 807 CCGG C^CGG
HindIII 960 AAGCTT A^AGCTT
HpaII 807 CCGG C^CGG
HphI 790 TCACC GGTGA(8/7)
Ksp632I 826 CTCTTC CTCTTC(1/4)
MaeI 67 CTAG C^TAG
MspA1I 434 CAGCTG C[AC]G^C[GT]G
MspI 807 CCGG C^CGG
NciI 807 CCGGG CC^[GC]GG
NcoI 61 CCATGG C^CATGG
NdeI 252 CATATG CA^TATG
PflMI 499 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 921 GAGTC GAGTC(4/5)
PvuII 434 CAGCTG CAG^CTG
SfcI 1031 CTATAG C^T[GA][CT]AG
Van91I 499 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 331 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 147 ACATGT A^C[GA][CT]GT
718 ACATGT
BbsI 335 GTCTTC GAAGAC(2/6)
923 GTCTTC
BbvI 433 GCAGC GCAGC(8/12)
436 GCTGC
BpuAI 335 GTCTTC GAAGAC(2/6)
923 GTCTTC
BspLU11I 147 ACATGT A^CATGT
718 ACATGT
BsrI 426 CCAGT ACTGG(1/-1)
684 CCAGT
BstF5I 440 CATCC GGATG(2/0)
639 CATCC
Cfr13I 207 GGACC G^G[ACGT]CC
294 GGCCC
DpnII 55 GATC ^GATC
523 GATC
EcoT14I 61 CCATGG C^C[AT][AT]GG
317 CCTTGG
Fnu4HI 433 GCAGC GC^[ACGT]GC
436 GCTGC
FokI 440 CATCC GGATG(9/13)
639 CATCC
HincII 568 GTTGAC GT[CT]^[GA]AC
698 GTCAAC
HindII 568 GTTGAC GT[CT]^[GA]AC
698 GTCAAC
ItaI 433 GCAGC GC^[ACGT]GC
436 GCTGC
MboI 55 GATC ^GATC
523 GATC
MslI 168 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
603 CATCAGTGTG
MwoI 408 GCACCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
580 GCAAATGAGGC
NdeII 55 GATC ^GATC
523 GATC
NlaIV 322 GGTTCC GG[ACGT]^[ACGT]CC
724 GGCTCC
NspI 147 ACATGT [GA]CATG^[CT]
718 ACATGT
Sau3AI 55 GATC ^GATC
523 GATC
Sau96I 207 GGACC G^G[ACGT]CC
294 GGCCC
SfaNI 495 GATGC GCATC(5/9)
439 GCATC
SmlI 490 CTTAAG C^T[CT][GA]AG
553 CTCAAG
StyI 61 CCATGG C^C[AT][AT]GG
317 CCTTGG
TseI 433 GCAGC G^C[AT]GC
436 GCTGC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsmFI BssKI BstNI DdeI EcoRII
HaeIII HinfI MaeIII MboII MnlI
MseI MvaI NlaIII RsaI ScrFI
TfiI Tru9I Tsp45I Tsp509I TspRI