Restriction map for
pFN21AE6155
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI HphI KasI
KpnI Ksp632I KspI MamI MfeI
MluNI MroI MscI MseI MslI
MunI NarI NciI NcoI NdeI
NlaIII NotI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 150 GTAC GT^AC
AflIII 49 ACGCGT A^C[GA][CT]GT
Alw44I 91 GTGCAC G^TGCAC
ApaLI 91 GTGCAC G^TGCAC
AspHI 91 GTGCAC G[AT]GC[AT]^C
AvaI 32 CTCGGG C^[CT]CG[GA]G
BglII 185 AGATCT A^GATCT
BmyI 91 GTGCAC G[AGT]GC[ACT]^C
BpmI 143 CTCCAG CTGGAG(16/14)
BsiEI 179 CGGCCG CG[GA][CT]^CG
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
BsoBI 32 CTCGGG C^[CT]CG[GA]G
Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C
BspMI 248 ACCTGC ACCTGC(4/8)
BstYI 185 AGATCT [GA]^GATC[CT]
DpnII 186 GATC ^GATC
EagI 179 CGGCCG C^GGCCG
EclXI 179 CGGCCG C^GGCCG
Eco52I 179 CGGCCG C^GGCCG
EcoT14I 380 CCAAGG C^C[AT][AT]GG
HaeII 139 GGCGCT [GA]GCGC^[CT]
HindIII 286 AAGCTT A^AGCTT
HinfI 128 GAGTC G^A[ACGT]TC
MaeII 9 ACGT A^CGT
MaeIII 301 GTGAC ^GT[ACGT]AC
MboI 186 GATC ^GATC
MflI 185 AGATCT [GA]^GATC[CT]
MluI 49 ACGCGT A^CGCGT
MspA1I 158 CAGCGG C[AC]G^C[GT]G
NaeI 136 GCCGGC GCC^GGC
NdeII 186 GATC ^GATC
NgoMIV 136 GCCGGC G^CCGGC
NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC
NruI 213 TCGCGA TCG^CGA
PleI 128 GAGTC GAGTC(4/5)
PstI 250 CTGCAG CTGCA^G
RsaI 150 GTAC GT^AC
Sau3AI 186 GATC ^GATC
SfaNI 233 GCATC GCATC(5/9)
SfcI 250 CTGCAG C^T[GA][CT]AG
StyI 380 CCAAGG C^C[AT][AT]GG
Tsp45I 301 GTGAC ^GT[GC]AC
TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 185 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BsaJI 59 CCAGGG C^C[ACGT][ACGT]GG
380 CCAAGG
BseRI 100 CTCCTC GAGGAG(10/8)
274 GAGGAG
BsiYI 153 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
369 CCACCATAAGG
BslI 153 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
369 CCACCATAAGG
BsrFI 64 GCCGGT [GA]^CCGG[CT]
136 GCCGGC
BsrI 79 CCAGT ACTGG(1/-1)
145 CCAGT
Cfr10I 64 GCCGGT [GA]^CCGG[CT]
136 GCCGGC
Cfr13I 62 GGGCC G^G[ACGT]CC
134 GGGCC
DdeI 189 CTTAG C^T[ACGT]AG
353 CTAAG
EaeI 84 TGGCCG [CT]^GGCC[GA]
179 CGGCCG
FauI 230 CCCGC CCCGC(4/6)
240 CCCGC
HapII 65 CCGG C^CGG
137 CCGG
HpaII 65 CCGG C^CGG
137 CCGG
MspI 65 CCGG C^CGG
137 CCGG
NheI 195 GCTAGC G^CTAGC
255 GCTAGC
Sau96I 62 GGGCC G^G[ACGT]CC
134 GGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AccII AciI AluI BbsI BbvI
BfaI BpuAI BssKI BstF5I BstNI
BstUI Cac8I CfoI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I ItaI
MaeI MboII MnlI MvaI MvnI
MwoI ScrFI TseI