Restriction map for
pFN21AE1205
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HinfI KasI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 343 GGTACC G^GTACC
AccI 363 GTATAC GT^[AC][GT]AC
AciI 290 CCGC CCGC(-3/-1)
AcsI 189 GAATTC [GA]^AATT[CT]
AlwI 282 GATCC GGATC(4/5)
AlwNI 20 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 257 AGCGCT AGC^GCT
ApoI 189 GAATTC [GA]^AATT[CT]
Asp700I 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 343 GGTACC G^GTACC
BalI 320 TGGCCA TGG^CCA
BanI 343 GGTACC G^G[CT][GA]CC
BbvI 303 GCTGC GCAGC(8/12)
BcnI 310 CCCGG CC^[GC]GG
BpmI 273 CTCCAG CTGGAG(16/14)
Bsp1407I 125 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BsrGI 125 TGTACA T^GTACA
BsrI 157 ACTGG ACTGG(1/-1)
Bst1107I 363 GTATAC GTA^TAC
BstBI 211 TTCGAA TT^CGAA
BstNI 275 CCAGG CC^[AT]GG
BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 281 AGATCC [GA]^GATC[CT]
BstZ17I 363 GTATAC GTA^TAC
Cfr13I 243 GGCCC G^G[ACGT]CC
DdeI 14 CTAAG C^T[ACGT]AG
DpnII 282 GATC ^GATC
DraIII 328 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCATGG C^C[GA][CT]GG
EaeI 320 TGGCCA [CT]^GGCC[GA]
Eco47III 257 AGCGCT AGC^GCT
EcoRI 189 GAATTC G^AATTC
EcoRII 275 CCAGG ^CC[AT]GG
Fnu4HI 303 GCTGC GC^[ACGT]GC
HaeII 257 AGCGCT [GA]GCGC^[CT]
HapII 311 CCGG C^CGG
HgaI 297 GCGTC GACGC(5/10)
HincII 199 GTTAAC GT[CT]^[GA]AC
HindII 199 GTTAAC GT[CT]^[GA]AC
HpaI 199 GTTAAC GTT^AAC
HpaII 311 CCGG C^CGG
ItaI 303 GCTGC GC^[ACGT]GC
KpnI 343 GGTACC GGTAC^C
MboI 282 GATC ^GATC
MflI 281 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MseI 200 TTAA T^TAA
MspI 311 CCGG C^CGG
MvaI 275 CCAGG CC^[AT]GG
NciI 310 CCCGG CC^[GC]GG
NcoI 176 CCATGG C^CATGG
NdeII 282 GATC ^GATC
NspV 211 TTCGAA TT^CGAA
RcaI 185 TCATGA T^CATGA
Sau3AI 282 GATC ^GATC
Sau96I 243 GGCCC G^G[ACGT]CC
SfuI 211 TTCGAA TT^CGAA
SspBI 125 TGTACA T^GTACA
Tru9I 200 TTAA T^TAA
TseI 303 GCTGC G^C[AT]GC
Tsp509I 190 AATT ^AATT
TspRI 332 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 281 AGATCC [GA]^GATC[CT]
XmnI 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 126 GTAC GT^AC
344 GTAC
BsiYI 31 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGATACTGG
BslI 31 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGATACTGG
BssKI 275 CCAGG ^CC[ACGT]GG
310 CCCGG
BstF5I 167 CATCC GGATG(2/0)
75 GGATG
FokI 167 CATCC GGATG(9/13)
75 GGATG
HphI 147 TCACC GGTGA(8/7)
356 TCACC
MaeIII 55 GTGAC ^GT[ACGT]AC
355 GTCAC
MnlI 272 CCTC CCTC(7/6)
229 GAGG
NlaIII 177 CATG CATG^
186 CATG
NlaIV 40 GGTTCC GG[ACGT]^[ACGT]CC
343 GGTACC
RsaI 126 GTAC GT^AC
344 GTAC
ScrFI 275 CCAGG CC^[ACGT]GG
310 CCCGG
TaqI 212 TCGA T^CGA
262 TCGA
Tsp45I 55 GTGAC ^GT[GC]AC
355 GTCAC
TthHB8I 212 TCGA T^CGA
262 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BsaJI Cac8I CfoI EcoT14I
HaeIII HhaI HinP1I MboII MwoI
SfaNI StyI