Restriction map for
pFN21AE5431
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BciVI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HgaI
Hin1I HpaI KasI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NarI NciI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SalI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 287 ACATGT A^C[GA][CT]GT
ApaI 3 GGGCCC GGGCC^C
AspHI 853 GAGCAC G[AT]GC[AT]^C
BbsI 382 GAAGAC GAAGAC(2/6)
BcgI 513 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 183 TGATCA T^GATCA
BfaI 913 CTAG C^TAG
BglI 13 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 382 GAAGAC GAAGAC(2/6)
BsiHKAI 853 GAGCAC G[AT]GC[AT]^C
BspHI 186 TCATGA T^CATGA
BspLU11I 287 ACATGT A^CATGT
BspMI 354 GCAGGT ACCTGC(4/8)
BsrFI 18 GCCGGC [GA]^CCGG[CT]
BstAPI 573 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 751 TTCGAA TT^CGAA
BstXI 491 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
EcoRI 439 GAATTC G^AATTC
FauI 6 CCCGC CCCGC(4/6)
FbaI 183 TGATCA T^GATCA
HapII 19 CCGG C^CGG
HincII 298 GTTGAC GT[CT]^[GA]AC
HindII 298 GTTGAC GT[CT]^[GA]AC
HindIII 143 AAGCTT A^AGCTT
HpaII 19 CCGG C^CGG
KspI 50 CCGCGG CCGC^GG
MaeI 913 CTAG C^TAG
MseI 985 TTAA T^TAA
MslI 702 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 19 CCGG C^CGG
NaeI 18 GCCGGC GCC^GGC
NgoMIV 18 GCCGGC G^CCGGC
NspI 287 ACATGT [GA]CATG^[CT]
NspV 751 TTCGAA TT^CGAA
RcaI 186 TCATGA T^CATGA
SacII 50 CCGCGG CCGC^GG
SapI 826 GAAGAGC GCTCTTC(1/4)
SfuI 751 TTCGAA TT^CGAA
SspI 981 AATATT AAT^ATT
StuI 861 AGGCCT AGG^CCT
TaqI 752 TCGA T^CGA
TfiI 519 GATTC G^A[AT]TC
Tru9I 985 TTAA T^TAA
TthHB8I 752 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 8 CGCG CG^CG
51 CGCG
AlwI 282 GGATC GGATC(4/5)
867 GGATC
BanI 106 GGTGCC G^G[CT][GA]CC
907 GGCACC
BsaAI 154 TACGTG [CT]AC^GT[GA]
840 TACGTG
BseRI 28 CTCCTC GAGGAG(10/8)
1007 GAGGAG
BsgI 88 CTGCAC GTGCAG(16/14)
563 CTGCAC
BstUI 8 CGCG CG^CG
51 CGCG
CfoI 22 GCGC GCG^C
66 GCGC
HaeII 21 GGCGCT [GA]GCGC^[CT]
65 GGCGCT
HhaI 22 GCGC GCG^C
66 GCGC
HinP1I 22 GCGC G^CGC
66 GCGC
MvnI 8 CGCG CG^CG
51 CGCG
NcoI 217 CCATGG C^CATGG
1013 CCATGG
PflMI 479 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCATGAAGTGG
PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT]
784 GGGACCT
PshAI 48 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
121 GACCTTGGTC
SbfI 57 CCTGCAGG CCTGCA^GG
351 CCTGCAGG
SfaNI 36 GATGC GCATC(5/9)
739 GATGC
Sse8387I 57 CCTGCAGG CCTGCA^GG
351 CCTGCAGG
Van91I 479 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCATGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI Tsp45I Tsp509I
TspRI