Restriction map for
pFN21AE2458
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 736 CGCG CG^CG
AcyI 37 GACGCC G[GA]^CG[CT]C
AfaI 146 GTAC GT^AC
AflIII 128 ACATGT A^C[GA][CT]GT
AlwI 507 GGATC GGATC(4/5)
AlwNI 377 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 65 AGCGCT AGC^GCT
Asp700I 274 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 385 GAGCTC G[AT]GC[AT]^C
AspI 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 11 CCCGGG C^[CT]CG[GA]G
AvaII 720 GGACC G^G[AT]CC
BanI 463 GGCACC G^G[CT][GA]CC
BlpI 382 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC
BsaHI 37 GACGCC G[GA]^CG[CT]C
BsaI 404 GAGACC GGTCTC(1/5)
BsiHKAI 385 GAGCTC G[AT]GC[AT]^C
BsmAI 404 GAGAC GTCTC(1/5)
BsmFI 599 GGGAC GGGAC(10/14)
BsmI 230 GCATTC GAATGC(1/-1)
BsoBI 11 CCCGGG C^[CT]CG[GA]G
BspHI 161 TCATGA T^CATGA
BspLU11I 128 ACATGT A^CATGT
BspMI 54 GCAGGT ACCTGC(4/8)
BstNI 648 CCTGG CC^[AT]GG
BstUI 736 CGCG CG^CG
BstYI 507 GGATCT [GA]^GATC[CT]
CelII 382 GCTGAGC GC^T[ACGT]AGC
DpnII 508 GATC ^GATC
DraI 562 TTTAAA TTT^AAA
DraIII 178 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 65 AGCGCT AGC^GCT
EcoRII 648 CCTGG ^CC[AT]GG
FauI 713 GCGGG CCCGC(4/6)
Hin1I 37 GACGCC G[GA]^CG[CT]C
MaeII 761 ACGT A^CGT
MboI 508 GATC ^GATC
MflI 507 GGATCT [GA]^GATC[CT]
MseI 563 TTAA T^TAA
MvaI 648 CCTGG CC^[AT]GG
MvnI 736 CGCG CG^CG
NdeII 508 GATC ^GATC
NspI 128 ACATGT [GA]CATG^[CT]
PflFI 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 21 GACTC GAGTC(4/5)
PvuII 377 CAGCTG CAG^CTG
RcaI 161 TCATGA T^CATGA
RsaI 146 GTAC GT^AC
SacI 385 GAGCTC GAGCT^C
SapI 535 GCTCTTC GCTCTTC(1/4)
Sau3AI 508 GATC ^GATC
SmaI 11 CCCGGG CCC^GGG
TaqI 110 TCGA T^CGA
Tru9I 563 TTAA T^TAA
Tsp45I 752 GTCAC ^GT[GC]AC
Tth111I 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 110 TCGA T^CGA
XhoII 507 GGATCT [GA]^GATC[CT]
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
XmnI 274 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 171 AAATTT [GA]^AATT[CT]
779 AAATTC
ApoI 171 AAATTT [GA]^AATT[CT]
779 AAATTC
BanII 385 GAGCTC G[GA]GC[CT]^C
644 GAGCCC
BmyI 385 GAGCTC G[AGT]GC[ACT]^C
644 GAGCCC
BsaJI 11 CCCGGG C^C[ACGT][ACGT]GG
647 CCCTGG
Bsp1286I 385 GAGCTC G[AGT]GC[ACT]^C
644 GAGCCC
Cfr13I 655 GGCCC G^G[ACGT]CC
720 GGACC
EarI 536 CTCTTC CTCTTC(1/4)
97 GAAGAG
HaeII 29 GGCGCT [GA]GCGC^[CT]
65 AGCGCT
HaeIII 429 GGCC GG^CC
655 GGCC
HapII 12 CCGG C^CGG
297 CCGG
HpaII 12 CCGG C^CGG
297 CCGG
HphI 183 GGTGA GGTGA(8/7)
417 TCACC
Ksp632I 536 CTCTTC CTCTTC(1/4)
97 GAAGAG
MaeIII 89 GTTAC ^GT[ACGT]AC
752 GTCAC
MspI 12 CCGG C^CGG
297 CCGG
Sau96I 655 GGCCC G^G[ACGT]CC
720 GGACC
TfiI 528 GATTC G^A[AT]TC
741 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BsiYI
BslI BsrI BssKI BstF5I Cac8I
CfoI DdeI Fnu4HI FokI HgaI
HhaI HinP1I HinfI ItaI MboII
MnlI MslI MspA1I MwoI NciI
NlaIII NlaIV ScrFI SfaNI TseI
Tsp509I TspRI