Restriction map for
pFN21AE2217
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanII
BbrPI BbsI BbvCI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI RcaI RsrII SacI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 445 GTCGAC GT^[AC][GT]AC
AcyI 591 GGCGCC G[GA]^CG[CT]C
AocI 307 CCTCAGG CC^T[ACGT]AGG
BamHI 688 GGATCC G^GATCC
BanI 591 GGCGCC G^G[CT][GA]CC
BbeI 591 GGCGCC GGCGC^C
BbvI 516 GCTGC GCAGC(8/12)
BcgI 213 CGAGTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 111 GGATAC GTATCC(6/5)
BsaHI 591 GGCGCC G[GA]^CG[CT]C
BsgI 764 GTGCAG GTGCAG(16/14)
BsiEI 818 CGATCG CG[GA][CT]^CG
BssSI 211 CACGAG CACGAG(-5/-1)
BstNI 801 CCAGG CC^[AT]GG
Bsu36I 307 CCTCAGG CC^T[ACGT]AGG
CfoI 592 GCGC GCG^C
DraI 736 TTTAAA TTT^AAA
DraII 859 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 307 CCTCAGG CC^T[ACGT]AGG
EcoO109I 859 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 801 CCAGG ^CC[AT]GG
FauI 634 GCGGG CCCGC(4/6)
HaeII 591 GGCGCC [GA]GCGC^[CT]
HaeIII 831 GGCC GG^CC
HhaI 592 GCGC GCG^C
Hin1I 591 GGCGCC G[GA]^CG[CT]C
HinP1I 592 GCGC G^CGC
HincII 445 GTCGAC GT[CT]^[GA]AC
HindII 445 GTCGAC GT[CT]^[GA]AC
KasI 591 GGCGCC G^GCGCC
KspI 587 CCGCGG CCGC^GG
MslI 170 CATTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 801 CCAGG CC^[AT]GG
MwoI 510 GCCCCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 591 GGCGCC GG^CGCC
NcoI 419 CCATGG C^CATGG
NspI 329 GCATGT [GA]CATG^[CT]
PpuMI 859 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 861 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 818 CGATCG CGAT^CG
SacII 587 CCGCGG CCGC^GG
SalI 445 GTCGAC G^TCGAC
ScaI 251 AGTACT AGT^ACT
SfaNI 298 GATGC GCATC(5/9)
SfoI 591 GGCGCC GGC^GCC
SspI 793 AATATT AAT^ATT
TseI 516 GCTGC G^C[AT]GC
XcmI 419 CCATGGAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 588 CGCG CG^CG
816 CGCG
AcsI 86 AAATTC [GA]^AATT[CT]
722 AAATTT
ApoI 86 AAATTC [GA]^AATT[CT]
722 AAATTT
Asp700I 394 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
790 GAAAATATTC
AvaII 860 GGACC G^G[AT]CC
867 GGTCC
BcnI 498 CCGGG CC^[GC]GG
864 CCCGG
BfaI 190 CTAG C^TAG
703 CTAG
BpmI 340 CTGGAG CTGGAG(16/14)
379 CTGGAG
BstUI 588 CGCG CG^CG
816 CGCG
BstYI 688 GGATCC [GA]^GATC[CT]
698 AGATCC
Cfr13I 860 GGACC G^G[ACGT]CC
867 GGTCC
DsaI 419 CCATGG C^C[GA][CT]GG
587 CCGCGG
EcoT14I 224 CCAAGG C^C[AT][AT]GG
419 CCATGG
Fnu4HI 516 GCTGC GC^[ACGT]GC
589 GCGGC
HapII 498 CCGG C^CGG
865 CCGG
HgaI 467 GACGC GACGC(5/10)
814 GACGC
HpaII 498 CCGG C^CGG
865 CCGG
HphI 228 GGTGA GGTGA(8/7)
461 TCACC
ItaI 516 GCTGC GC^[ACGT]GC
589 GCGGC
MaeI 190 CTAG C^TAG
703 CTAG
MaeIII 354 GTTAC ^GT[ACGT]AC
452 GTTAC
MflI 688 GGATCC [GA]^GATC[CT]
698 AGATCC
MspI 498 CCGG C^CGG
865 CCGG
MvnI 588 CGCG CG^CG
816 CGCG
NciI 498 CCGGG CC^[GC]GG
864 CCCGG
PvuII 337 CAGCTG CAG^CTG
514 CAGCTG
Sau96I 860 GGACC G^G[ACGT]CC
867 GGTCC
StyI 224 CCAAGG C^C[AT][AT]GG
419 CCATGG
XhoII 688 GGATCC [GA]^GATC[CT]
698 AGATCC
XmnI 394 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
790 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI AlwI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I DdeI DpnII FokI HinfI
MboI MboII MnlI MseI MspA1I
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I