Restriction map for
pFN21AE1812
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MboI MfeI MflI MluI
MunI MvnI NaeI NarI NciI
NcoI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI Sau3AI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 114 TCCGGA T^CCGGA
ApaI 926 GGGCCC GGGCC^C
BanII 926 GGGCCC G[GA]GC[CT]^C
BbsI 865 GTCTTC GAAGAC(2/6)
BciVI 111 GTATCC GTATCC(6/5)
BpuAI 865 GTCTTC GAAGAC(2/6)
BsaBI 106 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 114 TCCGGA [AT]^CCGG[AT]
BseAI 114 TCCGGA T^CCGGA
BseRI 607 GAGGAG GAGGAG(10/8)
BspEI 114 TCCGGA T^CCGGA
BsrI 258 CCAGT ACTGG(1/-1)
BssSI 58 CTCGTG CACGAG(-5/-1)
DraI 348 TTTAAA TTT^AAA
DsaI 887 CCACGG C^C[GA][CT]GG
EarI 483 CTCTTC CTCTTC(1/4)
EcoRI 741 GAATTC G^AATTC
FauI 924 GCGGG CCCGC(4/6)
HapII 115 CCGG C^CGG
HpaII 115 CCGG C^CGG
Ksp632I 483 CTCTTC CTCTTC(1/4)
MamI 106 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 114 TCCGGA T^CCGGA
MslI 915 CATTAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 115 CCGG C^CGG
NdeI 66 CATATG CA^TATG
NlaIII 630 CATG CATG^
NlaIV 926 GGGCCC GG[ACGT]^[ACGT]CC
PflMI 9 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 482 GCTCTTC GCTCTTC(1/4)
SmlI 652 CTCAAG C^T[CT][GA]AG
Van91I 9 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 620 CCATTGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 329 GAATTT [GA]^AATT[CT]
741 GAATTC
AlwNI 588 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
599 CAGCTGCTG
ApoI 329 GAATTT [GA]^AATT[CT]
741 GAATTC
BalI 62 TGGCCA TGG^CCA
255 TGGCCA
BbvCI 881 CCTCAGC CCTCAGC(-5/-2)
604 GCTGAGG
BmyI 926 GGGCCC G[AGT]GC[ACT]^C
973 GTGCCC
BpmI 125 CTGGAG CTGGAG(16/14)
783 CTGGAG
BsmFI 545 GGGAC GGGAC(10/14)
689 GGGAC
Bsp1286I 926 GGGCCC G[AGT]GC[ACT]^C
973 GTGCCC
BspMI 421 ACCTGC ACCTGC(4/8)
750 GCAGGT
EaeI 62 TGGCCA [CT]^GGCC[GA]
255 TGGCCA
EcoT14I 182 CCTTGG C^C[AT][AT]GG
495 CCAAGG
MaeII 414 ACGT A^CGT
505 ACGT
MluNI 62 TGGCCA TGG^CCA
255 TGGCCA
MscI 62 TGGCCA TGG^CCA
255 TGGCCA
MseI 349 TTAA T^TAA
736 TTAA
PstI 527 CTGCAG CTGCA^G
978 CTGCAG
StyI 182 CCTTGG C^C[AT][AT]GG
495 CCAAGG
Tru9I 349 TTAA T^TAA
736 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MspA1I
MvaI MwoI PleI PvuII RsaI
Sau96I ScaI ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI