Restriction map for
pFN21AE2528
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI KpnI KspI MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1333 GACGTC GACGT^C
AccI 1118 GTCTAC GT^[AC][GT]AC
AccIII 148 TCCGGA T^CCGGA
AfaI 3 GTAC GT^AC
Asp700I 510 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 893 CTCGAG C^[CT]CG[GA]G
AvrII 846 CCTAGG C^CTAGG
BanI 1150 GGCGCC G^G[CT][GA]CC
BbeI 1150 GGCGCC GGCGC^C
BfaI 847 CTAG C^TAG
BlnI 846 CCTAGG C^CTAGG
BsaI 37 GAGACC GGTCTC(1/5)
BsaWI 148 TCCGGA [AT]^CCGG[AT]
BseAI 148 TCCGGA T^CCGGA
BseRI 1204 GAGGAG GAGGAG(10/8)
BsoBI 893 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2 TGTACA T^GTACA
BspEI 148 TCCGGA T^CCGGA
BsrBI 733 GAGCGG CCGCTC(-3/-3)
BsrGI 2 TGTACA T^GTACA
BssSI 836 CTCGTG CACGAG(-5/-1)
CfoI 1151 GCGC GCG^C
DraI 178 TTTAAA TTT^AAA
DrdI 253 GACTATAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 1150 GGCGCC [GA]GCGC^[CT]
HhaI 1151 GCGC GCG^C
HinP1I 1151 GCGC G^CGC
HindIII 555 AAGCTT A^AGCTT
HpaI 994 GTTAAC GTT^AAC
KasI 1150 GGCGCC G^GCGCC
MaeI 847 CTAG C^TAG
MroI 148 TCCGGA T^CCGGA
NarI 1150 GGCGCC GG^CGCC
PaeR7I 893 CTCGAG C^TCGAG
PpuMI 183 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 3 GTAC GT^AC
SacI 882 GAGCTC GAGCT^C
SfoI 1150 GGCGCC GGC^GCC
SspBI 2 TGTACA T^GTACA
Tsp45I 108 GTCAC ^GT[GC]AC
XhoI 893 CTCGAG C^TCGAG
XmnI 510 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 513 GAATTT [GA]^AATT[CT]
700 AAATTC
AcyI 1150 GGCGCC G[GA]^CG[CT]C
1333 GACGTC
AflIII 535 ACATGT A^C[GA][CT]GT
1292 ACATGT
ApaI 398 GGGCCC GGGCC^C
1107 GGGCCC
ApoI 513 GAATTT [GA]^AATT[CT]
700 AAATTC
AspHI 862 GTGCTC G[AT]GC[AT]^C
882 GAGCTC
BcnI 446 CCCGG CC^[GC]GG
719 CCCGG
BpmI 730 CTGGAG CTGGAG(16/14)
1070 CTGGAG
BsaHI 1150 GGCGCC G[GA]^CG[CT]C
1333 GACGTC
BsiHKAI 862 GTGCTC G[AT]GC[AT]^C
882 GAGCTC
BsmFI 185 GTCCC GGGAC(10/14)
1197 GTCCC
BspLU11I 535 ACATGT A^CATGT
1292 ACATGT
BsrDI 234 CATTGC GCAATG(2/0)
282 GCAATG
BstYI 440 GGATCT [GA]^GATC[CT]
800 GGATCT
DraII 183 AGGTCCC [GA]G^G[ACGT]CC[CT]
397 GGGGCCC
EcoO109I 183 AGGTCCC [GA]G^G[ACGT]CC[CT]
397 GGGGCCC
FauI 296 CCCGC CCCGC(4/6)
1256 CCCGC
Hin1I 1150 GGCGCC G[GA]^CG[CT]C
1333 GACGTC
HincII 262 GTCAAC GT[CT]^[GA]AC
994 GTTAAC
HindII 262 GTCAAC GT[CT]^[GA]AC
994 GTTAAC
MaeII 197 ACGT A^CGT
1334 ACGT
MflI 440 GGATCT [GA]^GATC[CT]
800 GGATCT
NciI 446 CCCGG CC^[GC]GG
719 CCCGG
NspI 535 ACATGT [GA]CATG^[CT]
1292 ACATGT
SmlI 666 CTTGAG C^T[CT][GA]AG
893 CTCGAG
XhoII 440 GGATCT [GA]^GATC[CT]
800 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I