Restriction map for
pFN21AE1662
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccII 496 CGCG CG^CG
AhdI 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 106 GGGCCC GGGCC^C
Asp700I 381 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 216 GCTGAGG CCTCAGC(-5/-2)
BciVI 332 GTATCC GTATCC(6/5)
BsaI 508 GAGACC GGTCTC(1/5)
BsgI 525 CTGCAC GTGCAG(16/14)
BsrFI 423 GCCGGT [GA]^CCGG[CT]
BssHII 495 GCGCGC G^CGCGC
BstUI 496 CGCG CG^CG
Cfr10I 423 GCCGGT [GA]^CCGG[CT]
DraIII 127 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 338 CCGTGG C^C[GA][CT]GG
Eam1105I 142 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 345 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 256 GTCAAC GT[CT]^[GA]AC
HindII 256 GTCAAC GT[CT]^[GA]AC
MaeIII 150 GTCAC ^GT[ACGT]AC
MslI 513 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 496 CGCG CG^CG
SfcI 306 CTACAG C^T[GA][CT]AG
TaqI 197 TCGA T^CGA
Tsp45I 150 GTCAC ^GT[GC]AC
TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 197 TCGA T^CGA
XmnI 381 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 132 GGTGCC G^G[CT][GA]CC
359 GGTGCC
BanII 69 GAGCCC G[GA]GC[CT]^C
106 GGGCCC
BmyI 69 GAGCCC G[AGT]GC[ACT]^C
106 GGGCCC
BsmAI 508 GAGAC GTCTC(1/5)
294 GTCTC
Bsp1286I 69 GAGCCC G[AGT]GC[ACT]^C
106 GGGCCC
BsrI 5 ACTGG ACTGG(1/-1)
138 ACTGG
BstF5I 155 GGATG GGATG(2/0)
272 GGATG
BstXI 41 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
512 CCATCGCTGTGG
DdeI 217 CTGAG C^T[ACGT]AG
228 CTCAG
DraII 106 GGGCCCC [GA]G^G[ACGT]CC[CT]
480 AGGGCCT
EcoO109I 106 GGGCCCC [GA]G^G[ACGT]CC[CT]
480 AGGGCCT
FokI 155 GGATG GGATG(9/13)
272 GGATG
HapII 349 CCGG C^CGG
424 CCGG
HpaII 349 CCGG C^CGG
424 CCGG
MspA1I 214 CAGCTG C[AC]G^C[GT]G
265 CAGCTG
MspI 349 CCGG C^CGG
424 CCGG
PvuII 214 CAGCTG CAG^CTG
265 CAGCTG
SexAI 128 ACCAGGT A^CC[AT]GGT
143 ACCTGGT
SfaNI 311 GCATC GCATC(5/9)
533 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BspMI BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HphI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI