Restriction map for
pFN21AE1887
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbvCI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoT14I
EcoT22I FauI FseI HindIII KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 67 GACGTC GACGT^C
AccI 652 GTCTAC GT^[AC][GT]AC
AcyI 67 GACGTC G[GA]^CG[CT]C
AlwNI 424 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 802 GAGCAC G[AT]GC[AT]^C
AvaII 60 GGACC G^G[AT]CC
AviII 1025 TGCGCA TGC^GCA
BalI 134 TGGCCA TGG^CCA
BanII 984 GAGCCC G[GA]GC[CT]^C
BbrPI 1099 CACGTG CAC^GTG
BcgI 104 GCATGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 778 AGATCT A^GATCT
BsaAI 1099 CACGTG [CT]AC^GT[GA]
BsaHI 67 GACGTC G[GA]^CG[CT]C
BsiHKAI 802 GAGCAC G[AT]GC[AT]^C
BsmFI 83 GGGAC GGGAC(10/14)
BstEII 198 GGTCACC G^GT[ACGT]ACC
BstPI 198 GGTCACC G^GT[ACGT]ACC
BstYI 778 AGATCT [GA]^GATC[CT]
DrdI 61 GACCACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 606 CTCTTC CTCTTC(1/4)
EcoNI 732 CCTTAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 198 GGTCACC G^GT[ACGT]ACC
EcoRV 1094 GATATC GAT^ATC
FspI 1025 TGCGCA TGC^GCA
HaeII 22 AGCGCC [GA]GCGC^[CT]
Hin1I 67 GACGTC G[GA]^CG[CT]C
HincII 874 GTTAAC GT[CT]^[GA]AC
HindII 874 GTTAAC GT[CT]^[GA]AC
HpaI 874 GTTAAC GTT^AAC
Ksp632I 606 CTCTTC CTCTTC(1/4)
MflI 778 AGATCT [GA]^GATC[CT]
MluNI 134 TGGCCA TGG^CCA
MscI 134 TGGCCA TGG^CCA
MseI 875 TTAA T^TAA
NspI 885 GCATGT [GA]CATG^[CT]
PmaCI 1099 CACGTG CAC^GTG
PmlI 1099 CACGTG CAC^GTG
PstI 190 CTGCAG CTGCA^G
SfcI 190 CTGCAG C^T[GA][CT]AG
SmlI 814 CTCAAG C^T[CT][GA]AG
TaqI 182 TCGA T^CGA
TfiI 1198 GATTC G^A[AT]TC
Tru9I 875 TTAA T^TAA
TthHB8I 182 TCGA T^CGA
XhoII 778 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 686 AGCT AG^CT
1013 AGCT
AlwI 891 GATCC GGATC(4/5)
387 GGATC
AvaI 10 CCCGGG C^[CT]CG[GA]G
449 CCCGGG
BanI 36 GGCACC G^G[CT][GA]CC
258 GGTGCC
BclI 455 TGATCA T^GATCA
638 TGATCA
BfaI 250 CTAG C^TAG
1184 CTAG
BsaI 364 GAGACC GGTCTC(1/5)
562 GAGACC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
449 CCCGGG
BspMI 565 ACCTGC ACCTGC(4/8)
1028 GCAGGT
BsrI 222 ACTGG ACTGG(1/-1)
515 ACTGG
DdeI 707 CTGAG C^T[ACGT]AG
733 CTTAG
EaeI 134 TGGCCA [CT]^GGCC[GA]
353 CGGCCA
FbaI 455 TGATCA T^GATCA
638 TGATCA
HgaI 186 GCGTC GACGC(5/10)
347 GCGTC
MaeI 250 CTAG C^TAG
1184 CTAG
PleI 770 GACTC GAGTC(4/5)
1228 GACTC
SmaI 10 CCCGGG CCC^GGG
449 CCCGGG
StuI 483 AGGCCT AGG^CCT
1043 AGGCCT
XmaCI 10 CCCGGG C^CCGGG
449 CCCGGG
XmaI 10 CCCGGG C^CCGGG
449 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrDI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I TspRI