Restriction map for
pFN21AE6292
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuII
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 751 GGTACC G^GTACC
AccII 333 CGCG CG^CG
AflII 759 CTTAAG C^TTAAG
Asp718I 751 GGTACC G^GTACC
AvaI 519 CTCGAG C^[CT]CG[GA]G
BbsI 156 GAAGAC GAAGAC(2/6)
BciVI 476 GTATCC GTATCC(6/5)
BfaI 619 CTAG C^TAG
BfrI 759 CTTAAG C^TTAAG
BpmI 319 CTCCAG CTGGAG(16/14)
BpuAI 156 GAAGAC GAAGAC(2/6)
BsaI 175 GGTCTC GGTCTC(1/5)
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsgI 359 CTGCAC GTGCAG(16/14)
BsiEI 583 CGATCG CG[GA][CT]^CG
BsmAI 176 GTCTC GTCTC(1/5)
BsmFI 58 GGGAC GGGAC(10/14)
BsoBI 519 CTCGAG C^[CT]CG[GA]G
Bsp1407I 128 TGTACA T^GTACA
BsrGI 128 TGTACA T^GTACA
BstNI 13 CCTGG CC^[AT]GG
BstUI 333 CGCG CG^CG
EarI 241 CTCTTC CTCTTC(1/4)
EcoRI 707 GAATTC G^AATTC
EcoRII 13 CCTGG ^CC[AT]GG
HgaI 595 GCGTC GACGC(5/10)
HphI 140 TCACC GGTGA(8/7)
KpnI 751 GGTACC GGTAC^C
Ksp632I 241 CTCTTC CTCTTC(1/4)
KspI 332 CCGCGG CCGC^GG
MaeI 619 CTAG C^TAG
MvaI 13 CCTGG CC^[AT]GG
MvnI 333 CGCG CG^CG
NaeI 395 GCCGGC GCC^GGC
NcoI 510 CCATGG C^CATGG
NgoMIV 395 GCCGGC G^CCGGC
PaeR7I 519 CTCGAG C^TCGAG
PleI 515 GACTC GAGTC(4/5)
PpuMI 337 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 326 CTGCAG CTGCA^G
PvuI 583 CGATCG CGAT^CG
SacII 332 CCGCGG CCGC^GG
ScaI 523 AGTACT AGT^ACT
SspBI 128 TGTACA T^GTACA
TfiI 819 GATTC G^A[AT]TC
XcmI 407 CCATGTATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 519 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 659 GAATTT [GA]^AATT[CT]
707 GAATTC
ApoI 659 GAATTT [GA]^AATT[CT]
707 GAATTC
BanI 196 GGCACC G^G[CT][GA]CC
751 GGTACC
BglI 494 GCCCCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
835 GCCAATGGGGC
BmyI 195 GGGCAC G[AGT]GC[ACT]^C
842 GGGCAC
Bsp1286I 195 GGGCAC G[AGT]GC[ACT]^C
842 GGGCAC
BsrFI 199 ACCGGC [GA]^CCGG[CT]
395 GCCGGC
BstAPI 352 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
826 GCAATCCCTGC
BstF5I 18 CATCC GGATG(2/0)
489 GGATG
Cfr10I 199 ACCGGC [GA]^CCGG[CT]
395 GCCGGC
DsaI 332 CCGCGG C^C[GA][CT]GG
510 CCATGG
EcoNI 126 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
480 CCTTAAAAAGG
EcoT14I 497 CCTTGG C^C[AT][AT]GG
510 CCATGG
FauI 43 GCGGG CCCGC(4/6)
334 GCGGG
FokI 18 CATCC GGATG(9/13)
489 GGATG
HinfI 515 GACTC G^A[ACGT]TC
819 GATTC
MaeII 115 ACGT A^CGT
221 ACGT
MspA1I 41 CAGCGG C[AC]G^C[GT]G
332 CCGCGG
SfaNI 108 GATGC GCATC(5/9)
17 GCATC
SfcI 326 CTGCAG C^T[GA][CT]AG
400 CTACAG
StyI 497 CCTTGG C^C[AT][AT]GG
510 CCATGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BsaJI BsiYI BslI BsrI
BssKI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I Fnu4HI HaeIII HapII
HpaII ItaI MboI MboII MnlI
MseI MspI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SmlI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I