Restriction map for
pFN21AE6360
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StyI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 248 GGCGCC G[GA]^CG[CT]C
Aor51HI 432 AGCGCT AGC^GCT
ApaI 235 GGGCCC GGGCC^C
Asp700I 660 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 490 CCCGGG C^[CT]CG[GA]G
BanII 235 GGGCCC G[GA]GC[CT]^C
BbeI 248 GGCGCC GGCGC^C
BbvCI 152 CCTCAGC CCTCAGC(-5/-2)
BlpI 771 GCTGAGC GC^T[ACGT]AGC
BpmI 515 CTCCAG CTGGAG(16/14)
Bpu1102I 771 GCTGAGC GC^T[ACGT]AGC
BsaHI 248 GGCGCC G[GA]^CG[CT]C
BsiEI 129 CGGCCG CG[GA][CT]^CG
BsoBI 490 CCCGGG C^[CT]CG[GA]G
Bsp1407I 611 TGTACA T^GTACA
BsrGI 611 TGTACA T^GTACA
BsrI 506 CCAGT ACTGG(1/-1)
BssSI 763 CTCGTG CACGAG(-5/-1)
BstAPI 520 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 771 GCTGAGC GC^T[ACGT]AGC
DpnII 309 GATC ^GATC
EagI 129 CGGCCG C^GGCCG
EarI 664 CTCTTC CTCTTC(1/4)
EclXI 129 CGGCCG C^GGCCG
Eco47III 432 AGCGCT AGC^GCT
Eco52I 129 CGGCCG C^GGCCG
HgaI 420 GCGTC GACGC(5/10)
Hin1I 248 GGCGCC G[GA]^CG[CT]C
HincII 865 GTCAAC GT[CT]^[GA]AC
HindII 865 GTCAAC GT[CT]^[GA]AC
HinfI 693 GAATC G^A[ACGT]TC
HphI 306 GGTGA GGTGA(8/7)
KasI 248 GGCGCC G^GCGCC
Ksp632I 664 CTCTTC CTCTTC(1/4)
MaeIII 323 GTTAC ^GT[ACGT]AC
MboI 309 GATC ^GATC
NarI 248 GGCGCC GG^CGCC
NdeII 309 GATC ^GATC
NspI 705 GCATGC [GA]CATG^[CT]
PstI 898 CTGCAG CTGCA^G
Sau3AI 309 GATC ^GATC
SfcI 898 CTGCAG C^T[GA][CT]AG
SfiI 227 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 248 GGCGCC GGC^GCC
SmaI 490 CCCGGG CCC^GGG
SmlI 361 CTCAAG C^T[CT][GA]AG
SphI 705 GCATGC GCATG^C
SspBI 611 TGTACA T^GTACA
TfiI 693 GAATC G^A[AT]TC
XcmI 777 CCAGGCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 490 CCCGGG C^CCGGG
XmaI 490 CCCGGG C^CCGGG
XmnI 660 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 382 GTGCTC G[AT]GC[AT]^C
874 GTGCTC
AvaII 56 GGACC G^G[AT]CC
330 GGACC
BalI 923 TGGCCA TGG^CCA
944 TGGCCA
BcnI 490 CCCGG CC^[GC]GG
491 CCGGG
BfaI 559 CTAG C^TAG
697 CTAG
BglI 138 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
228 GCCCCAGGGGC
BseRI 150 CTCCTC GAGGAG(10/8)
571 CTCCTC
BsiHKAI 382 GTGCTC G[AT]GC[AT]^C
874 GTGCTC
BsmFI 38 GTCCC GGGAC(10/14)
509 GTCCC
BspHI 626 TCATGA T^CATGA
809 TCATGA
BsrDI 104 CATTGC GCAATG(2/0)
929 CATTGC
BstF5I 12 GGATG GGATG(2/0)
751 GGATG
FauI 50 CCCGC CCCGC(4/6)
454 GCGGG
FokI 12 GGATG GGATG(9/13)
751 GGATG
MaeI 559 CTAG C^TAG
697 CTAG
MluNI 923 TGGCCA TGG^CCA
944 TGGCCA
MscI 923 TGGCCA TGG^CCA
944 TGGCCA
MslI 78 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
375 CACAGCTGTG
NciI 490 CCCGG CC^[GC]GG
491 CCGGG
PvuII 377 CAGCTG CAG^CTG
521 CAGCTG
RcaI 626 TCATGA T^CATGA
809 TCATGA
SexAI 302 ACCTGGT A^CC[AT]GGT
344 ACCAGGT
SfaNI 63 GATGC GCATC(5/9)
827 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
StuI TseI TspRI