Restriction map for
pFN21AE1833
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 872 GGCGCC G[GA]^CG[CT]C
Alw44I 879 GTGCAC G^TGCAC
ApaLI 879 GTGCAC G^TGCAC
Asp700I 75 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 879 GTGCAC G[AT]GC[AT]^C
AvaII 94 GGTCC G^G[AT]CC
AviII 1006 TGCGCA TGC^GCA
BanII 470 GAGCCC G[GA]GC[CT]^C
BbeI 872 GGCGCC GGCGC^C
BbvCI 712 GCTGAGG CCTCAGC(-5/-2)
BcnI 603 CCGGG CC^[GC]GG
BpmI 281 CTGGAG CTGGAG(16/14)
BsaHI 872 GGCGCC G[GA]^CG[CT]C
BseRI 154 GAGGAG GAGGAG(10/8)
BsiHKAI 879 GTGCAC G[AT]GC[AT]^C
BsmI 49 GCATTC GAATGC(1/-1)
Bsp1407I 290 TGTACA T^GTACA
BsrFI 499 GCCGGC [GA]^CCGG[CT]
BsrGI 290 TGTACA T^GTACA
BstAPI 49 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 325 TTCGAA TT^CGAA
Cfr10I 499 GCCGGC [GA]^CCGG[CT]
DraII 93 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 372 CCATGG C^C[GA][CT]GG
EcoNI 1035 CCTGGGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 93 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 598 GAATTC G^AATTC
FauI 709 CCCGC CCCGC(4/6)
FspI 1006 TGCGCA TGC^GCA
Hin1I 872 GGCGCC G[GA]^CG[CT]C
KasI 872 GGCGCC G^GCGCC
MaeII 861 ACGT A^CGT
NaeI 499 GCCGGC GCC^GGC
NarI 872 GGCGCC GG^CGCC
NciI 603 CCGGG CC^[GC]GG
NcoI 372 CCATGG C^CATGG
NdeI 36 CATATG CA^TATG
NgoMIV 499 GCCGGC G^CCGGC
NspV 325 TTCGAA TT^CGAA
PflMI 1029 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 284 GAGTC GAGTC(4/5)
PpuMI 93 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 205 GCTCTTC GCTCTTC(1/4)
SfoI 872 GGCGCC GGC^GCC
SfuI 325 TTCGAA TT^CGAA
SpeI 121 ACTAGT A^CTAGT
SspBI 290 TGTACA T^GTACA
Van91I 1029 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 428 CCAAACTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 75 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 380 GGATC GGATC(4/5)
422 GGATC
AlwNI 640 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1030 CAGTCCCTG
BfaI 122 CTAG C^TAG
728 CTAG
BsmAI 250 GAGAC GTCTC(1/5)
614 GAGAC
BsmFI 95 GTCCC GGGAC(10/14)
1032 GTCCC
BspMI 778 ACCTGC ACCTGC(4/8)
897 GCAGGT
BstF5I 845 CATCC GGATG(2/0)
462 GGATG
BstYI 380 GGATCT [GA]^GATC[CT]
422 GGATCT
DraIII 700 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
882 CACTGAGTG
EarI 206 CTCTTC CTCTTC(1/4)
904 GAAGAG
FokI 845 CATCC GGATG(9/13)
462 GGATG
HapII 500 CCGG C^CGG
603 CCGG
HpaII 500 CCGG C^CGG
603 CCGG
Ksp632I 206 CTCTTC CTCTTC(1/4)
904 GAAGAG
MaeI 122 CTAG C^TAG
728 CTAG
MflI 380 GGATCT [GA]^GATC[CT]
422 GGATCT
MslI 536 CATGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
845 CATCCAGGTG
MspI 500 CCGG C^CGG
603 CCGG
StuI 101 AGGCCT AGG^CCT
716 AGGCCT
TaqI 152 TCGA T^CGA
326 TCGA
TfiI 480 GAATC G^A[AT]TC
760 GAATC
Tsp45I 320 GTGAC ^GT[GC]AC
971 GTGAC
TthHB8I 152 TCGA T^CGA
326 TCGA
XhoII 380 GGATCT [GA]^GATC[CT]
422 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp509I TspRI