Restriction map for
pFN21AB6744
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII ItaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MspA1I MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
TseI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 360 CGCG CG^CG
AciI 73 GCGG CCGC(-3/-1)
AcsI 389 AAATTT [GA]^AATT[CT]
AfaI 292 GTAC GT^AC
AhdI 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 123 AGCT AG^CT
ApoI 389 AAATTT [GA]^AATT[CT]
AspEI 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 39 GTGCTC G[AT]GC[AT]^C
AvaII 15 GGTCC G^G[AT]CC
BalI 8 TGGCCA TGG^CCA
BamHI 77 GGATCC G^GATCC
BbsI 380 GTCTTC GAAGAC(2/6)
BmyI 39 GTGCTC G[AGT]GC[ACT]^C
BpuAI 380 GTCTTC GAAGAC(2/6)
BsgI 189 CTGCAC GTGCAG(16/14)
BsiHKAI 39 GTGCTC G[AT]GC[AT]^C
BsmI 173 GCATTC GAATGC(1/-1)
Bsp1286I 39 GTGCTC G[AGT]GC[ACT]^C
BsrI 155 CCAGT ACTGG(1/-1)
BstF5I 416 GGATG GGATG(2/0)
BstUI 360 CGCG CG^CG
CfoI 361 GCGC GCG^C
Cfr13I 15 GGTCC G^G[ACGT]CC
DdeI 221 CTCAG C^T[ACGT]AG
EaeI 8 TGGCCA [CT]^GGCC[GA]
Eam1105I 153 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 375 GAAGAG CTCTTC(1/4)
FauI 73 GCGGG CCCGC(4/6)
FokI 416 GGATG GGATG(9/13)
HhaI 361 GCGC GCG^C
HinP1I 361 GCGC G^CGC
HinfI 378 GAGTC G^A[ACGT]TC
HphI 273 GGTGA GGTGA(8/7)
Ksp632I 375 GAAGAG CTCTTC(1/4)
MaeIII 151 GTGAC ^GT[ACGT]AC
MluNI 8 TGGCCA TGG^CCA
MscI 8 TGGCCA TGG^CCA
MslI 136 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 360 CGCG CG^CG
MwoI 352 GCAGGCAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 11 CCATGG C^CATGG
NlaIV 77 GGATCC GG[ACGT]^[ACGT]CC
PleI 378 GAGTC GAGTC(4/5)
PstI 138 CTGCAG CTGCA^G
RsaI 292 GTAC GT^AC
Sau96I 15 GGTCC G^G[ACGT]CC
ScaI 291 AGTACT AGT^ACT
SfaNI 108 GATGC GCATC(5/9)
SfcI 138 CTGCAG C^T[GA][CT]AG
Tsp45I 151 GTGAC ^GT[GC]AC
Tsp509I 390 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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BsiYI 68 CCTTGGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCAAACCACGG
BslI 68 CCTTGGCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCAAACCACGG
BssKI 54 CCAGG ^CC[ACGT]GG
81 CCTGG
BstNI 54 CCAGG CC^[AT]GG
81 CCTGG
BstYI 77 GGATCC [GA]^GATC[CT]
117 AGATCC
Cac8I 49 GCAGGC GC[ACGT]^[ACGT]GC
352 GCAGGC
DpnII 78 GATC ^GATC
118 GATC
EcoRII 54 CCAGG ^CC[AT]GG
81 CCTGG
EcoT14I 11 CCATGG C^C[AT][AT]GG
68 CCTTGG
HaeIII 9 GGCC GG^CC
52 GGCC
MboI 78 GATC ^GATC
118 GATC
MflI 77 GGATCC [GA]^GATC[CT]
117 AGATCC
MvaI 54 CCAGG CC^[AT]GG
81 CCTGG
NdeII 78 GATC ^GATC
118 GATC
Sau3AI 78 GATC ^GATC
118 GATC
ScrFI 54 CCAGG CC^[ACGT]GG
81 CCTGG
StyI 11 CCATGG C^C[AT][AT]GG
68 CCTTGG
XhoII 77 GGATCC [GA]^GATC[CT]
117 AGATCC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AlwI BsaJI BsmAI DsaI MboII
MnlI NlaIII TspRI