Restriction map for
pFN21AE1918
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BciVI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 189 CGCG CG^CG
AflIII 695 ACATGT A^C[GA][CT]GT
Alw44I 1350 GTGCAC G^TGCAC
AlwI 432 GGATC GGATC(4/5)
ApaI 996 GGGCCC GGGCC^C
ApaLI 1350 GTGCAC G^TGCAC
Asp700I 298 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 229 CCTAGG C^CTAGG
BbsI 552 GTCTTC GAAGAC(2/6)
BbvCI 919 GCTGAGG CCTCAGC(-5/-2)
BcgI 560 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 362 CCCGG CC^[GC]GG
BglII 931 AGATCT A^GATCT
BlnI 229 CCTAGG C^CTAGG
BpuAI 552 GTCTTC GAAGAC(2/6)
BsaI 383 GAGACC GGTCTC(1/5)
BseRI 1233 CTCCTC GAGGAG(10/8)
Bsp1407I 476 TGTACA T^GTACA
BspLU11I 695 ACATGT A^CATGT
BspMI 1260 ACCTGC ACCTGC(4/8)
BsrGI 476 TGTACA T^GTACA
BstEII 224 GGTCACC G^GT[ACGT]ACC
BstPI 224 GGTCACC G^GT[ACGT]ACC
BstUI 189 CGCG CG^CG
BstYI 931 AGATCT [GA]^GATC[CT]
CfoI 190 GCGC GCG^C
DraII 867 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 768 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1199 CCATGG C^C[GA][CT]GG
EcoO109I 867 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 224 GGTCACC G^GT[ACGT]ACC
FauI 187 CCCGC CCCGC(4/6)
HapII 363 CCGG C^CGG
HhaI 190 GCGC GCG^C
HinP1I 190 GCGC G^CGC
HpaII 363 CCGG C^CGG
MaeII 6 ACGT A^CGT
MfeI 1310 CAATTG C^AATTG
MflI 931 AGATCT [GA]^GATC[CT]
MspI 363 CCGG C^CGG
MunI 1310 CAATTG C^AATTG
MvnI 189 CGCG CG^CG
NciI 362 CCCGG CC^[GC]GG
NcoI 1199 CCATGG C^CATGG
NdeI 284 CATATG CA^TATG
NspI 695 ACATGT [GA]CATG^[CT]
PpuMI 867 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 345 GCTCTTC GCTCTTC(1/4)
SexAI 386 ACCAGGT A^CC[AT]GGT
SfaNI 735 GCATC GCATC(5/9)
SspBI 476 TGTACA T^GTACA
StuI 856 AGGCCT AGG^CCT
TaqI 1137 TCGA T^CGA
TthHB8I 1137 TCGA T^CGA
XhoII 931 AGATCT [GA]^GATC[CT]
XmnI 298 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 140 GGTCC G^G[AT]CC
868 GGTCC
BsmAI 167 GAGAC GTCTC(1/5)
383 GAGAC
BsrI 1106 ACTGG ACTGG(1/-1)
1189 CCAGT
BstF5I 736 CATCC GGATG(2/0)
924 GGATG
Cac8I 175 GCTGGC GC[ACGT]^[ACGT]GC
372 GCAGGC
EarI 346 CTCTTC CTCTTC(1/4)
910 GAAGAG
FokI 736 CATCC GGATG(9/13)
924 GGATG
Ksp632I 346 CTCTTC CTCTTC(1/4)
910 GAAGAG
MslI 154 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1174 CATCGGTATG
MwoI 94 GCTCAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
103 GCTGCTAGTGC
PstI 148 CTGCAG CTGCA^G
235 CTGCAG
SacI 575 GAGCTC GAGCT^C
1060 GAGCTC
SfcI 148 CTGCAG C^T[GA][CT]AG
235 CTGCAG
SmlI 460 CTCAAG C^T[CT][GA]AG
1278 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI