Restriction map for
pFN21AE2592
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DrdI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HpaI KpnI Ksp632I MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 97 GGCGCC G[GA]^CG[CT]C
Alw44I 174 GTGCAC G^TGCAC
Aor51HI 23 AGCGCT AGC^GCT
ApaLI 174 GTGCAC G^TGCAC
BbeI 97 GGCGCC GGCGC^C
BbsI 388 GAAGAC GAAGAC(2/6)
BcgI 56 GCAGGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 407 CTAG C^TAG
BpmI 181 CTGGAG CTGGAG(16/14)
BpuAI 388 GAAGAC GAAGAC(2/6)
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsgI 54 GTGCAG GTGCAG(16/14)
BsmFI 310 GGGAC GGGAC(10/14)
BspDI 484 ATCGAT AT^CGAT
BspMI 609 GCAGGT ACCTGC(4/8)
BsrDI 332 GCAATG GCAATG(2/0)
BssSI 659 CTCGTG CACGAG(-5/-1)
BstAPI 167 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 484 ATCGAT AT^CGAT
DraIII 168 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4 CCGCGG C^C[GA][CT]GG
Eco47III 23 AGCGCT AGC^GCT
FauI 40 GCGGG CCCGC(4/6)
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HindIII 627 AAGCTT A^AGCTT
KasI 97 GGCGCC G^GCGCC
KspI 4 CCGCGG CCGC^GG
MaeI 407 CTAG C^TAG
MaeIII 206 GTCAC ^GT[ACGT]AC
MseI 508 TTAA T^TAA
MslI 208 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 4 CCGCGG C[AC]G^C[GT]G
NarI 97 GGCGCC GG^CGCC
SacII 4 CCGCGG CCGC^GG
SfoI 97 GGCGCC GGC^GCC
SmlI 518 CTCAAG C^T[CT][GA]AG
TfiI 442 GAATC G^A[AT]TC
Tru9I 508 TTAA T^TAA
Tsp45I 206 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 319 AAATTT [GA]^AATT[CT]
337 GAATTT
ApoI 319 AAATTT [GA]^AATT[CT]
337 GAATTT
AspHI 174 GTGCAC G[AT]GC[AT]^C
425 GTGCTC
AvaII 14 GGTCC G^G[AT]CC
88 GGACC
BcnI 10 CCGGG CC^[GC]GG
91 CCCGG
BsiEI 7 CGGCCG CG[GA][CT]^CG
32 CGGCCG
BsiHKAI 174 GTGCAC G[AT]GC[AT]^C
425 GTGCTC
BsrI 78 ACTGG ACTGG(1/-1)
199 ACTGG
BstYI 234 AGATCC [GA]^GATC[CT]
540 GGATCT
EaeI 7 CGGCCG [CT]^GGCC[GA]
32 CGGCCG
EagI 7 CGGCCG C^GGCCG
32 CGGCCG
EclXI 7 CGGCCG C^GGCCG
32 CGGCCG
Eco52I 7 CGGCCG C^GGCCG
32 CGGCCG
HaeII 23 AGCGCT [GA]GCGC^[CT]
97 GGCGCC
HapII 10 CCGG C^CGG
92 CCGG
HpaII 10 CCGG C^CGG
92 CCGG
HphI 192 GGTGA GGTGA(8/7)
110 TCACC
MflI 234 AGATCC [GA]^GATC[CT]
540 GGATCT
MspI 10 CCGG C^CGG
92 CCGG
NciI 10 CCGGG CC^[GC]GG
91 CCCGG
PleI 495 GACTC GAGTC(4/5)
419 GAGTC
XcmI 170 CCAAGTGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
189 CCAGGTGATTACTGG
XhoII 234 AGATCC [GA]^GATC[CT]
540 GGATCT
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI AlwI BanI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI MboI MboII MnlI MvaI
MvnI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI TaqI TseI Tsp509I
TspRI TthHB8I