Restriction map for
pFN21AE1644
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HindIII HinfI
HphI KasI KpnI KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI NarI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI TspRI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 270 CGCG CG^CG
AciI 260 CCGC CCGC(-3/-1)
AcyI 443 GGCGTC G[GA]^CG[CT]C
AluI 318 AGCT AG^CT
Asp700I 45 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 170 GGACC G^G[AT]CC
BanI 326 GGTGCC G^G[CT][GA]CC
BmyI 327 GTGCCC G[AGT]GC[ACT]^C
BsaHI 443 GGCGTC G[GA]^CG[CT]C
BsaJI 330 CCCAGG C^C[ACGT][ACGT]GG
BseRI 378 GAGGAG GAGGAG(10/8)
BsiYI 256 CCCACCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 256 CCCACCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 199 GTCTC GTCTC(1/5)
BsmBI 198 CGTCTC CGTCTC(1/5)
BsmFI 169 GGGAC GGGAC(10/14)
Bsp1286I 327 GTGCCC G[AGT]GC[ACT]^C
BsrFI 262 GCCGGC [GA]^CCGG[CT]
BssKI 331 CCAGG ^CC[ACGT]GG
BstNI 331 CCAGG CC^[AT]GG
BstUI 270 CGCG CG^CG
CfoI 269 GCGC GCG^C
Cfr10I 262 GCCGGC [GA]^CCGG[CT]
Cfr13I 170 GGACC G^G[ACGT]CC
EaeI 214 CGGCCA [CT]^GGCC[GA]
EarI 375 GAAGAG CTCTTC(1/4)
EcoRII 331 CCAGG ^CC[AT]GG
HaeIII 215 GGCC GG^CC
HapII 263 CCGG C^CGG
HhaI 269 GCGC GCG^C
Hin1I 443 GGCGTC G[GA]^CG[CT]C
HinP1I 269 GCGC G^CGC
HincII 78 GTTAAC GT[CT]^[GA]AC
HindII 78 GTTAAC GT[CT]^[GA]AC
HpaI 78 GTTAAC GTT^AAC
HpaII 263 CCGG C^CGG
Ksp632I 375 GAAGAG CTCTTC(1/4)
MnlI 378 GAGG CCTC(7/6)
MspI 263 CCGG C^CGG
MvaI 331 CCAGG CC^[AT]GG
MvnI 270 CGCG CG^CG
NaeI 262 GCCGGC GCC^GGC
NgoMIV 262 GCCGGC G^CCGGC
NlaIII 297 CATG CATG^
Sau96I 170 GGACC G^G[ACGT]CC
ScaI 133 AGTACT AGT^ACT
ScrFI 331 CCAGG CC^[ACGT]GG
TaqI 456 TCGA T^CGA
Tsp509I 428 AATT ^AATT
TthHB8I 456 TCGA T^CGA
XcmI 401 CCAATAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 45 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AfaI 134 GTAC GT^AC
179 GTAC
BsrDI 220 CATTGC GCAATG(2/0)
367 GCAATG
Cac8I 262 GCCGGC GC[ACGT]^[ACGT]GC
276 GCTCGC
HgaI 271 GCGTC GACGC(5/10)
444 GCGTC
MseI 79 TTAA T^TAA
116 TTAA
NlaIV 169 GGGACC GG[ACGT]^[ACGT]CC
326 GGTGCC
RsaI 134 GTAC GT^AC
179 GTAC
Tru9I 79 TTAA T^TAA
116 TTAA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
BbvI DdeI Fnu4HI ItaI MaeIII
MboII MwoI TseI Tsp45I