Restriction map for
pFN21AE0643
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MroI MseI MunI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 282 GTAC GT^AC
Aor51HI 1033 AGCGCT AGC^GCT
AscI 607 GGCGCGCC GG^CGCGCC
AspHI 421 GTGCTC G[AT]GC[AT]^C
BalI 1025 TGGCCA TGG^CCA
BbsI 455 GTCTTC GAAGAC(2/6)
BpuAI 455 GTCTTC GAAGAC(2/6)
BsaI 73 GGTCTC GGTCTC(1/5)
BseRI 1150 GAGGAG GAGGAG(10/8)
BsiHKAI 421 GTGCTC G[AT]GC[AT]^C
BsrI 980 ACTGG ACTGG(1/-1)
BssSI 415 CTCGTG CACGAG(-5/-1)
BstAPI 375 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 56 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 412 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1057 CTCTTC CTCTTC(1/4)
Eco47III 1033 AGCGCT AGC^GCT
FseI 466 GGCCGGCC GGCCGG^CC
HinfI 556 GACTC G^A[ACGT]TC
HphI 1209 TCACC GGTGA(8/7)
Ksp632I 1057 CTCTTC CTCTTC(1/4)
MaeII 592 ACGT A^CGT
MluNI 1025 TGGCCA TGG^CCA
MscI 1025 TGGCCA TGG^CCA
NlaIII 1213 CATG CATG^
NotI 949 GCGGCCGC GC^GGCCGC
PaeR7I 433 CTCGAG C^TCGAG
PflMI 411 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 556 GACTC GAGTC(4/5)
PpuMI 353 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 282 GTAC GT^AC
SbfI 684 CCTGCAGG CCTGCA^GG
SfaNI 388 GCATC GCATC(5/9)
SmaI 1160 CCCGGG CCC^GGG
SmlI 433 CTCGAG C^T[CT][GA]AG
Sse8387I 684 CCTGCAGG CCTGCA^GG
Tsp45I 306 GTCAC ^GT[GC]AC
Tsp509I 24 AATT ^AATT
Van91I 411 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 347 CCACGGGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 433 CTCGAG C^TCGAG
XmaCI 1160 CCCGGG C^CCGGG
XmaI 1160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 691 GTCTAC GT^[AC][GT]AC
1051 GTCTAC
AflIII 284 ACGCGT A^C[GA][CT]GT
695 ACGCGT
AlwI 6 GGATC GGATC(4/5)
656 GGATC
AlwNI 376 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
874 CAGAGCCTG
AvaI 433 CTCGAG C^[CT]CG[GA]G
1160 CCCGGG
BbeI 36 GGCGCC GGCGC^C
400 GGCGCC
BsgI 259 GTGCAG GTGCAG(16/14)
871 GTGCAG
BsmAI 977 GAGAC GTCTC(1/5)
74 GTCTC
BsoBI 433 CTCGAG C^[CT]CG[GA]G
1160 CCCGGG
BspMI 683 ACCTGC ACCTGC(4/8)
687 GCAGGT
BstF5I 153 CATCC GGATG(2/0)
389 CATCC
BstYI 6 GGATCT [GA]^GATC[CT]
656 GGATCT
DpnII 7 GATC ^GATC
657 GATC
EcoT14I 86 CCTTGG C^C[AT][AT]GG
866 CCAAGG
FokI 153 CATCC GGATG(9/13)
389 CATCC
KasI 36 GGCGCC G^GCGCC
400 GGCGCC
KspI 800 CCGCGG CCGC^GG
971 CCGCGG
MaeIII 306 GTCAC ^GT[ACGT]AC
733 GTTAC
MboI 7 GATC ^GATC
657 GATC
MflI 6 GGATCT [GA]^GATC[CT]
656 GGATCT
MluI 284 ACGCGT A^CGCGT
695 ACGCGT
NarI 36 GGCGCC GG^CGCC
400 GGCGCC
NdeII 7 GATC ^GATC
657 GATC
SacII 800 CCGCGG CCGC^GG
971 CCGCGG
Sau3AI 7 GATC ^GATC
657 GATC
SfoI 36 GGCGCC GGC^GCC
400 GGCGCC
SgrAI 32 CGCCGGCG C[GA]^CCGG[CT]G
439 CGCCGGCG
StyI 86 CCTTGG C^C[AT][AT]GG
866 CCAAGG
TaqI 434 TCGA T^CGA
834 TCGA
TspRI 185 CAGTG CA[GC]TG[ACGT][ACGT]^
515 CACTG
TthHB8I 434 TCGA T^CGA
834 TCGA
XhoII 6 GGATCT [GA]^GATC[CT]
656 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIV PstI
PvuII Sau96I ScrFI SfcI TseI