Restriction map for
pFN21AE6737
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BsaI BseAI
BsiWI BsmAI BsmBI BspDI BspEI
BsrBI BsrDI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoT22I
FbaI FseI FspI KpnI KspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I StuI Tth111I XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcyI 71 GGCGCC G[GA]^CG[CT]C
AflIII 1706 ACATGT A^C[GA][CT]GT
ApaI 1147 GGGCCC GGGCC^C
AvaI 67 CCCGGG C^[CT]CG[GA]G
BalI 203 TGGCCA TGG^CCA
BbeI 71 GGCGCC GGCGC^C
BbsI 2309 GAAGAC GAAGAC(2/6)
BglII 1941 AGATCT A^GATCT
BlpI 1735 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1735 GCTGAGC GC^T[ACGT]AGC
BpuAI 2309 GAAGAC GAAGAC(2/6)
BsaAI 1116 CACGTA [CT]AC^GT[GA]
BsaHI 71 GGCGCC G[GA]^CG[CT]C
BsaWI 798 TCCGGT [AT]^CCGG[AT]
BseRI 2110 GAGGAG GAGGAG(10/8)
BsgI 647 CTGCAC GTGCAG(16/14)
BsiEI 90 CGGCCG CG[GA][CT]^CG
BsoBI 67 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2457 TGTACA T^GTACA
BspLU11I 1706 ACATGT A^CATGT
BspMI 277 GCAGGT ACCTGC(4/8)
BsrFI 2564 ACCGGC [GA]^CCGG[CT]
BsrGI 2457 TGTACA T^GTACA
BssHII 14 GCGCGC G^CGCGC
BssSI 755 CTCGTG CACGAG(-5/-1)
BstEII 972 GGTCACC G^GT[ACGT]ACC
BstPI 972 GGTCACC G^GT[ACGT]ACC
CelII 1735 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2564 ACCGGC [GA]^CCGG[CT]
DrdI 964 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 90 CGGCCG C^GGCCG
EclXI 90 CGGCCG C^GGCCG
Eco52I 90 CGGCCG C^GGCCG
EcoO65I 972 GGTCACC G^GT[ACGT]ACC
EcoRV 2019 GATATC GAT^ATC
HgaI 2086 GACGC GACGC(5/10)
Hin1I 71 GGCGCC G[GA]^CG[CT]C
HincII 1710 GTTAAC GT[CT]^[GA]AC
HindII 1710 GTTAAC GT[CT]^[GA]AC
HindIII 845 AAGCTT A^AGCTT
HpaI 1710 GTTAAC GTT^AAC
KasI 71 GGCGCC G^GCGCC
MluNI 203 TGGCCA TGG^CCA
MscI 203 TGGCCA TGG^CCA
NarI 71 GGCGCC GG^CGCC
PflMI 991 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1916 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 19 CAGCTG CAG^CTG
SacI 247 GAGCTC GAGCT^C
SfoI 71 GGCGCC GGC^GCC
SmaI 67 CCCGGG CCC^GGG
SphI 468 GCATGC GCATG^C
SspBI 2457 TGTACA T^GTACA
SwaI 438 ATTTAAAT ATTT^AAAT
Van91I 991 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1023 TCTAGA T^CTAGA
XcmI 1640 CCATTAGACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 67 CCCGGG C^CCGGG
XmaI 67 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 15 CGCG CG^CG
77 CGCG
AcsI 436 GAATTT [GA]^AATT[CT]
503 AAATTC
ApoI 436 GAATTT [GA]^AATT[CT]
503 AAATTC
AspHI 247 GAGCTC G[AT]GC[AT]^C
868 GTGCTC
BamHI 1241 GGATCC G^GATCC
1281 GGATCC
BanI 71 GGCGCC G^G[CT][GA]CC
628 GGTGCC
BsiHKAI 247 GAGCTC G[AT]GC[AT]^C
868 GTGCTC
BspHI 1803 TCATGA T^CATGA
2093 TCATGA
BstAPI 18 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
601 GCACTGGCTGC
BstUI 15 CGCG CG^CG
77 CGCG
DraI 439 TTTAAA TTT^AAA
636 TTTAAA
DsaI 1055 CCATGG C^C[GA][CT]GG
1448 CCATGG
EaeI 90 CGGCCG [CT]^GGCC[GA]
203 TGGCCA
EarI 2060 GAAGAG CTCTTC(1/4)
2430 GAAGAG
HaeII 71 GGCGCC [GA]GCGC^[CT]
2076 GGCGCT
Ksp632I 2060 GAAGAG CTCTTC(1/4)
2430 GAAGAG
MaeII 692 ACGT A^CGT
1117 ACGT
MspA1I 19 CAGCTG C[AC]G^C[GT]G
52 CCGCTG
MvnI 15 CGCG CG^CG
77 CGCG
NcoI 1055 CCATGG C^CATGG
1448 CCATGG
NspI 468 GCATGC [GA]CATG^[CT]
1706 ACATGT
RcaI 1803 TCATGA T^CATGA
2093 TCATGA
SspI 1357 AATATT AAT^ATT
2228 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaBI BsaJI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI