Restriction map for
pFN21AE1665
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BanII
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 122 CGCG CG^CG
AcsI 391 AAATTC [GA]^AATT[CT]
ApoI 391 AAATTC [GA]^AATT[CT]
AspHI 139 GTGCTC G[AT]GC[AT]^C
AvaI 234 CCCGGG C^[CT]CG[GA]G
BamHI 267 GGATCC G^GATCC
BanI 313 GGCACC G^G[CT][GA]CC
BbsI 423 GTCTTC GAAGAC(2/6)
BbvCI 240 GCTGAGG CCTCAGC(-5/-2)
BglI 350 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 139 GTGCTC G[AGT]GC[ACT]^C
BpuAI 423 GTCTTC GAAGAC(2/6)
BseRI 28 CTCCTC GAGGAG(10/8)
BsgI 38 CTGCAC GTGCAG(16/14)
BsiHKAI 139 GTGCTC G[AT]GC[AT]^C
BsmI 507 GAATGC GAATGC(1/-1)
BsoBI 234 CCCGGG C^[CT]CG[GA]G
Bsp1286I 139 GTGCTC G[AGT]GC[ACT]^C
BspMI 340 ACCTGC ACCTGC(4/8)
BsrI 351 CCAGT ACTGG(1/-1)
BssSI 31 CTCGTG CACGAG(-5/-1)
BstAPI 101 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 122 CGCG CG^CG
BstYI 267 GGATCC [GA]^GATC[CT]
Cfr13I 232 GGCCC G^G[ACGT]CC
DpnII 268 GATC ^GATC
DraIII 43 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 50 TGGCCG [CT]^GGCC[GA]
HgaI 8 GCGTC GACGC(5/10)
HphI 277 GGTGA GGTGA(8/7)
MboI 268 GATC ^GATC
MflI 267 GGATCC [GA]^GATC[CT]
MseI 541 TTAA T^TAA
MvnI 122 CGCG CG^CG
NdeII 268 GATC ^GATC
PstI 173 CTGCAG CTGCA^G
SapI 3 GAAGAGC GCTCTTC(1/4)
Sau3AI 268 GATC ^GATC
Sau96I 232 GGCCC G^G[ACGT]CC
SexAI 44 ACCTGGT A^CC[AT]GGT
SfcI 173 CTGCAG C^T[GA][CT]AG
SmaI 234 CCCGGG CCC^GGG
TaqI 143 TCGA T^CGA
Tru9I 541 TTAA T^TAA
TthHB8I 143 TCGA T^CGA
XhoII 267 GGATCC [GA]^GATC[CT]
XmaCI 234 CCCGGG C^CCGGG
XmaI 234 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 268 GATCC GGATC(4/5)
267 GGATC
BpmI 57 CTGGAG CTGGAG(16/14)
467 CTGGAG
BsaI 337 GAGACC GGTCTC(1/5)
518 GGTCTC
BsaJI 234 CCCGGG C^C[ACGT][ACGT]GG
235 CCGGGG
BsiYI 253 CCTTCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCGTAATGAGG
BslI 253 CCTTCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCGTAATGAGG
BsmFI 373 GGGAC GGGAC(10/14)
305 GTCCC
BstF5I 216 GGATG GGATG(2/0)
323 GGATG
BstNI 45 CCTGG CC^[AT]GG
56 CCTGG
Cac8I 36 GCCTGC GC[ACGT]^[ACGT]GC
119 GCCCGC
CfoI 123 GCGC GCG^C
170 GCGC
DdeI 207 CTCAG C^T[ACGT]AG
241 CTGAG
EarI 3 GAAGAG CTCTTC(1/4)
280 GAAGAG
EcoRII 45 CCTGG ^CC[AT]GG
56 CCTGG
FauI 120 CCCGC CCCGC(4/6)
180 GCGGG
FokI 216 GGATG GGATG(9/13)
323 GGATG
HaeIII 51 GGCC GG^CC
232 GGCC
HapII 235 CCGG C^CGG
515 CCGG
HhaI 123 GCGC GCG^C
170 GCGC
HinP1I 123 GCGC G^CGC
170 GCGC
HpaII 235 CCGG C^CGG
515 CCGG
Ksp632I 3 GAAGAG CTCTTC(1/4)
280 GAAGAG
MspI 235 CCGG C^CGG
515 CCGG
MvaI 45 CCTGG CC^[AT]GG
56 CCTGG
NlaIV 267 GGATCC GG[ACGT]^[ACGT]CC
313 GGCACC
SfaNI 359 GCATC GCATC(5/9)
463 GCATC
SspI 407 AATATT AAT^ATT
478 AATATT
Tsp45I 95 GTGAC ^GT[GC]AC
365 GTGAC
Tsp509I 67 AATT ^AATT
392 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BbvI BcnI BsmAI BssKI
Fnu4HI ItaI MaeIII MboII MnlI
MwoI NciI NlaIII ScrFI TseI
TspRI