Restriction map for
pFN21AE5811
##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsaI BseAI BsiWI BsmBI BsmI
BspDI BspEI BsrDI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NarI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1640 GACGTC GACGT^C
AccI 1953 GTCTAC GT^[AC][GT]AC
AflIII 1250 ACATGT A^C[GA][CT]GT
AgeI 1995 ACCGGT A^CCGGT
Alw44I 2299 GTGCAC G^TGCAC
ApaI 256 GGGCCC GGGCC^C
ApaLI 2299 GTGCAC G^TGCAC
BamHI 1437 GGATCC G^GATCC
BanI 118 GGCACC G^G[CT][GA]CC
BcgI 1133 GCATGATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 258 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1995 ACCGGT [AT]^CCGG[AT]
BspHI 572 TCATGA T^CATGA
BspLU11I 1250 ACATGT A^CATGT
BsrBI 2138 CCGCTC CCGCTC(-3/-3)
BstBI 241 TTCGAA TT^CGAA
DrdI 2010 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1941 GAATTC G^AATTC
FseI 92 GGCCGGCC GGCCGG^CC
MaeII 1641 ACGT A^CGT
NcoI 520 CCATGG C^CATGG
NdeI 863 CATATG CA^TATG
NspV 241 TTCGAA TT^CGAA
PflMI 1191 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1995 ACCGGT A^CCGGT
PpuMI 1712 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 572 TCATGA T^CATGA
SapI 2596 GCTCTTC GCTCTTC(1/4)
SfuI 241 TTCGAA TT^CGAA
SgrAI 1994 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1864 CTCAAG C^T[CT][GA]AG
SspI 1342 AATATT AAT^ATT
Van91I 1191 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 976 CCACCAGATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 1192 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2588 CAGCAGCTG
Asp700I 725 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1339 GAAAATATTC
AvaI 310 CCCGGG C^[CT]CG[GA]G
2339 CCCGGG
BclI 569 TGATCA T^GATCA
635 TGATCA
BfaI 469 CTAG C^TAG
1771 CTAG
BlpI 1128 GCTGAGC GC^T[ACGT]AGC
1461 GCTGAGC
Bpu1102I 1128 GCTGAGC GC^T[ACGT]AGC
1461 GCTGAGC
BsiEI 95 CGGCCG CG[GA][CT]^CG
101 CGGCCG
BsoBI 310 CCCGGG C^[CT]CG[GA]G
2339 CCCGGG
Bsp1407I 2000 TGTACA T^GTACA
2121 TGTACA
BsrGI 2000 TGTACA T^GTACA
2121 TGTACA
BstYI 1437 GGATCC [GA]^GATC[CT]
1821 GGATCT
CelII 1128 GCTGAGC GC^T[ACGT]AGC
1461 GCTGAGC
DraI 651 TTTAAA TTT^AAA
996 TTTAAA
EagI 95 CGGCCG C^GGCCG
101 CGGCCG
EclXI 95 CGGCCG C^GGCCG
101 CGGCCG
Eco52I 95 CGGCCG C^GGCCG
101 CGGCCG
FbaI 569 TGATCA T^GATCA
635 TGATCA
HaeII 110 AGCGCC [GA]GCGC^[CT]
2316 GGCGCT
KspI 98 CCGCGG CCGC^GG
2102 CCGCGG
MaeI 469 CTAG C^TAG
1771 CTAG
MflI 1437 GGATCC [GA]^GATC[CT]
1821 GGATCT
PshAI 2457 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2528 GACAGATGTC
SacII 98 CCGCGG CCGC^GG
2102 CCGCGG
SfaNI 912 GATGC GCATC(5/9)
541 GCATC
SmaI 310 CCCGGG CCC^GGG
2339 CCCGGG
SspBI 2000 TGTACA T^GTACA
2121 TGTACA
XhoII 1437 GGATCC [GA]^GATC[CT]
1821 GGATCT
XmaCI 310 CCCGGG C^CCGGG
2339 CCCGGG
XmaI 310 CCCGGG C^CCGGG
2339 CCCGGG
XmnI 725 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1339 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I