Restriction map for
pFN21AE3763
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BanII BbeI
BbrPI BbsI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoT22I FseI HaeII
HapII HpaI HpaII KasI KpnI
KspI MaeI MaeII MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 383 GGCGTC G[GA]^CG[CT]C
AocI 865 CCTGAGG CC^T[ACGT]AGG
AspHI 936 GTGCTC G[AT]GC[AT]^C
AviII 2 TGCGCA TGC^GCA
BanI 106 GGCACC G^G[CT][GA]CC
BbvCI 1528 GCTGAGG CCTCAGC(-5/-2)
BclI 1089 TGATCA T^GATCA
BpmI 18 CTGGAG CTGGAG(16/14)
BsaHI 383 GGCGTC G[GA]^CG[CT]C
BseRI 326 GAGGAG GAGGAG(10/8)
BsgI 572 CTGCAC GTGCAG(16/14)
BsiHKAI 936 GTGCTC G[AT]GC[AT]^C
BsmAI 319 GTCTC GTCTC(1/5)
BsmFI 1459 GGGAC GGGAC(10/14)
BsmI 486 GAATGC GAATGC(1/-1)
BspDI 1499 ATCGAT AT^CGAT
BspMI 780 GCAGGT ACCTGC(4/8)
Bsu36I 865 CCTGAGG CC^T[ACGT]AGG
CfoI 3 GCGC GCG^C
ClaI 1499 ATCGAT AT^CGAT
DraI 741 TTTAAA TTT^AAA
DraII 842 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1613 GACCTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 72 CGGCCA [CT]^GGCC[GA]
Eco81I 865 CCTGAGG CC^T[ACGT]AGG
EcoO109I 842 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 814 GATATC GAT^ATC
FauI 60 GCGGG CCCGC(4/6)
FbaI 1089 TGATCA T^GATCA
FspI 2 TGCGCA TGC^GCA
HgaI 384 GCGTC GACGC(5/10)
HhaI 3 GCGC GCG^C
Hin1I 383 GGCGTC G[GA]^CG[CT]C
HinP1I 3 GCGC G^CGC
HindIII 238 AAGCTT A^AGCTT
MfeI 1288 CAATTG C^AATTG
MspA1I 957 CAGCTG C[AC]G^C[GT]G
MunI 1288 CAATTG C^AATTG
PflMI 1609 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 842 AGGACCT [GA]G^G[AT]CC[CT]
PstI 778 CTGCAG CTGCA^G
PvuII 957 CAGCTG CAG^CTG
SalI 1244 GTCGAC G^TCGAC
SexAI 272 ACCAGGT A^CC[AT]GGT
SspI 737 AATATT AAT^ATT
SwaI 740 ATTTAAAT ATTT^AAAT
TspRI 630 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1609 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1244 GTCGAC GT^[AC][GT]AC
1376 GTCTAC
AciI 216 CCGC CCGC(-3/-1)
60 GCGG
AvaII 438 GGTCC G^G[AT]CC
843 GGACC
BbvI 571 GCTGC GCAGC(8/12)
777 GCTGC
BmyI 936 GTGCTC G[AGT]GC[ACT]^C
1151 GGGCAC
BsaBI 1087 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1478 GATCGGGATC
BsaJI 1147 CCAAGG C^C[ACGT][ACGT]GG
1197 CCAAGG
Bsp1286I 936 GTGCTC G[AGT]GC[ACT]^C
1151 GGGCAC
BsrI 145 ACTGG ACTGG(1/-1)
1413 ACTGG
Cac8I 604 GCAGGC GC[ACGT]^[ACGT]GC
1153 GCACGC
EcoT14I 1147 CCAAGG C^C[AT][AT]GG
1197 CCAAGG
Fnu4HI 571 GCTGC GC^[ACGT]GC
777 GCTGC
HincII 1244 GTCGAC GT[CT]^[GA]AC
1597 GTTGAC
HindII 1244 GTCGAC GT[CT]^[GA]AC
1597 GTTGAC
ItaI 571 GCTGC GC^[ACGT]GC
777 GCTGC
MamI 1087 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1478 GATCGGGATC
NdeI 430 CATATG CA^TATG
772 CATATG
SfcI 778 CTGCAG C^T[GA][CT]AG
1378 CTACAG
SmlI 235 CTCAAG C^T[CT][GA]AG
1535 CTCAAG
StyI 1147 CCAAGG C^C[AT][AT]GG
1197 CCAAGG
TseI 571 GCTGC G^C[AT]GC
777 GCTGC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BamHI BsiYI BslI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII FokI HaeIII
HinfI HphI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I Tsp45I
Tsp509I TthHB8I XhoII