Restriction map for
pFN21AE9567
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BseRI
BsgI BsiWI BsmI Bsp1407I BspDI
BspHI BspMI BsrGI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MluI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 1075 TCCGGA T^CCGGA
AcsI 1236 GAATTT [GA]^AATT[CT]
AflIII 557 ACATGT A^C[GA][CT]GT
AlwI 801 GATCC GGATC(4/5)
AlwNI 265 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1399 CCTAAGG CC^T[ACGT]AGG
ApoI 1236 GAATTT [GA]^AATT[CT]
Asp700I 947 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1276 GAAGAC GAAGAC(2/6)
BfaI 970 CTAG C^TAG
BpuAI 1276 GAAGAC GAAGAC(2/6)
BsaWI 1075 TCCGGA [AT]^CCGG[AT]
BseAI 1075 TCCGGA T^CCGGA
BsmBI 151 GAGACG CGTCTC(1/5)
BsmFI 1059 GTCCC GGGAC(10/14)
BspEI 1075 TCCGGA T^CCGGA
BspLU11I 557 ACATGT A^CATGT
BsrDI 822 CATTGC GCAATG(2/0)
BsrFI 44 ACCGGC [GA]^CCGG[CT]
BssSI 862 CTCGTG CACGAG(-5/-1)
BstEII 222 GGTGACC G^GT[ACGT]ACC
BstPI 222 GGTGACC G^GT[ACGT]ACC
BstYI 800 AGATCC [GA]^GATC[CT]
Bsu36I 1399 CCTAAGG CC^T[ACGT]AGG
Cfr10I 44 ACCGGC [GA]^CCGG[CT]
DraII 1057 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 692 CCGCGG C^C[GA][CT]GG
Eco81I 1399 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1057 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 222 GGTGACC G^GT[ACGT]ACC
EcoT14I 1224 CCTTGG C^C[AT][AT]GG
HgaI 433 GCGTC GACGC(5/10)
HincII 880 GTCAAC GT[CT]^[GA]AC
HindII 880 GTCAAC GT[CT]^[GA]AC
KspI 692 CCGCGG CCGC^GG
MaeI 970 CTAG C^TAG
MflI 800 AGATCC [GA]^GATC[CT]
MroI 1075 TCCGGA T^CCGGA
PflMI 384 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1057 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 487 CTGCAG CTGCA^G
SacI 1407 GAGCTC GAGCT^C
SacII 692 CCGCGG CCGC^GG
SapI 297 GCTCTTC GCTCTTC(1/4)
SmlI 33 CTTGAG C^T[CT][GA]AG
SphI 635 GCATGC GCATG^C
StyI 1224 CCTTGG C^C[AT][AT]GG
Van91I 384 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 800 AGATCC [GA]^GATC[CT]
XmnI 947 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 764 GGCGCC G[GA]^CG[CT]C
793 GGCGCC
Aor51HI 347 AGCGCT AGC^GCT
1070 AGCGCT
AvaII 521 GGACC G^G[AT]CC
1058 GGTCC
BalI 110 TGGCCA TGG^CCA
356 TGGCCA
BbeI 764 GGCGCC GGCGC^C
793 GGCGCC
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
750 CCTCAGC
BsaAI 234 CACGTA [CT]AC^GT[GA]
388 TACGTG
BsaHI 764 GGCGCC G[GA]^CG[CT]C
793 GGCGCC
BsaI 1312 GAGACC GGTCTC(1/5)
469 GGTCTC
BsrBI 859 CCGCTC CCGCTC(-3/-3)
1377 GAGCGG
BssHII 452 GCGCGC G^CGCGC
623 GCGCGC
BstAPI 323 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
424 GCAGTCTATGC
BstXI 496 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
827 CCACCTCCCTGG
DraIII 385 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
501 CACCTGGTG
EagI 689 CGGCCG C^GGCCG
695 CGGCCG
EclXI 689 CGGCCG C^GGCCG
695 CGGCCG
Eco47III 347 AGCGCT AGC^GCT
1070 AGCGCT
Eco52I 689 CGGCCG C^GGCCG
695 CGGCCG
EcoNI 1266 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1286 CCTCACTGAGG
Hin1I 764 GGCGCC G[GA]^CG[CT]C
793 GGCGCC
HinfI 1321 GACTC G^A[ACGT]TC
1339 GACTC
KasI 764 GGCGCC G^GCGCC
793 GGCGCC
MluNI 110 TGGCCA TGG^CCA
356 TGGCCA
MscI 110 TGGCCA TGG^CCA
356 TGGCCA
NarI 764 GGCGCC GG^CGCC
793 GGCGCC
NotI 688 GCGGCCGC GC^GGCCGC
694 GCGGCCGC
NspI 557 ACATGT [GA]CATG^[CT]
635 GCATGC
PleI 1321 GACTC GAGTC(4/5)
1339 GACTC
SexAI 104 ACCTGGT A^CC[AT]GGT
502 ACCTGGT
SfaNI 323 GCATC GCATC(5/9)
785 GCATC
SfcI 378 CTACAG C^T[GA][CT]AG
487 CTGCAG
SfoI 764 GGCGCC GGC^GCC
793 GGCGCC
TaqI 344 TCGA T^CGA
515 TCGA
TthHB8I 344 TCGA T^CGA
515 TCGA
XcmI 827 CCACCTCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
854 CCATCCCGCTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI BanI BanII BbvI BcnI
BglI BmyI BsaBI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SmaI TseI Tsp45I Tsp509I
TspRI XmaCI XmaI