Restriction map for
pFN21AE2147
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BseRI
BsgI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DdeI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MboII MfeI MflI MluI
MroI MseI MslI MspA1I MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 53 CGCG CG^CG
AcsI 158 GAATTC [GA]^AATT[CT]
AcyI 70 GGCGCC G[GA]^CG[CT]C
AgeI 35 ACCGGT A^CCGGT
AluI 276 AGCT AG^CT
ApoI 158 GAATTC [GA]^AATT[CT]
AspHI 275 GAGCTC G[AT]GC[AT]^C
AvaI 245 CTCGGG C^[CT]CG[GA]G
BanI 70 GGCGCC G^G[CT][GA]CC
BbeI 70 GGCGCC GGCGC^C
BcnI 104 CCGGG CC^[GC]GG
BpmI 349 CTCCAG CTGGAG(16/14)
BsaHI 70 GGCGCC G[GA]^CG[CT]C
BsaI 93 GGTCTC GGTCTC(1/5)
BsaWI 35 ACCGGT [AT]^CCGG[AT]
BsiEI 101 CGACCG CG[GA][CT]^CG
BsiHKAI 275 GAGCTC G[AT]GC[AT]^C
BsoBI 245 CTCGGG C^[CT]CG[GA]G
BstUI 53 CGCG CG^CG
EcoRI 158 GAATTC G^AATTC
HaeII 70 GGCGCC [GA]GCGC^[CT]
Hin1I 70 GGCGCC G[GA]^CG[CT]C
HinfI 243 GACTC G^A[ACGT]TC
KasI 70 GGCGCC G^GCGCC
MvnI 53 CGCG CG^CG
NaeI 23 GCCGGC GCC^GGC
NarI 70 GGCGCC GG^CGCC
NciI 104 CCGGG CC^[GC]GG
NgoMIV 23 GCCGGC G^CCGGC
NlaIII 332 CATG CATG^
NspI 331 ACATGC [GA]CATG^[CT]
PinAI 35 ACCGGT A^CCGGT
PleI 243 GACTC GAGTC(4/5)
SacI 275 GAGCTC GAGCT^C
SfcI 150 CTACAG C^T[GA][CT]AG
SfoI 70 GGCGCC GGC^GCC
Tsp45I 115 GTCAC ^GT[GC]AC
Tsp509I 159 AATT ^AATT
TspRI 123 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 126 TGGCCA TGG^CCA
282 TGGCCA
BanII 275 GAGCTC G[GA]GC[CT]^C
346 GGGCTC
BbvI 222 GCAGC GCAGC(8/12)
27 GCTGC
BmyI 275 GAGCTC G[AGT]GC[ACT]^C
346 GGGCTC
BsiYI 17 CCTTCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
60 CCTGCCCCTGG
BslI 17 CCTTCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
60 CCTGCCCCTGG
BsmAI 241 GAGAC GTCTC(1/5)
94 GTCTC
Bsp1286I 275 GAGCTC G[AGT]GC[ACT]^C
346 GGGCTC
BsrI 124 ACTGG ACTGG(1/-1)
351 CCAGT
CfoI 52 GCGC GCG^C
71 GCGC
EaeI 126 TGGCCA [CT]^GGCC[GA]
282 TGGCCA
FauI 90 GCGGG CCCGC(4/6)
269 GCGGG
HaeIII 127 GGCC GG^CC
283 GGCC
HhaI 52 GCGC GCG^C
71 GCGC
HinP1I 52 GCGC G^CGC
71 GCGC
HphI 186 GGTGA GGTGA(8/7)
249 GGTGA
MaeIII 115 GTCAC ^GT[ACGT]AC
338 GTTAC
MluNI 126 TGGCCA TGG^CCA
282 TGGCCA
MscI 126 TGGCCA TGG^CCA
282 TGGCCA
TseI 27 GCTGC G^C[AT]GC
222 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BsaJI BsrFI BssKI BstNI
Cac8I Cfr10I EcoRII Fnu4HI HapII
HpaII ItaI MnlI MspI MvaI
MwoI NlaIV ScrFI