Restriction map for
pFN21AE3321
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI AocI ApaI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BanII BbeI
BbvCI BcnI BglI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I CpoI DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HapII HgaI Hin1I
HindIII HpaII KasI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tsp45I Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflII 1149 CTTAAG C^TTAAG
Alw44I 81 GTGCAC G^TGCAC
AlwNI 1805 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 552 AGCGCT AGC^GCT
ApaLI 81 GTGCAC G^TGCAC
Asp700I 1881 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2635 TGGCCA TGG^CCA
BamHI 2692 GGATCC G^GATCC
BbrPI 1947 CACGTG CAC^GTG
BcgI 873 CGATTCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2132 GTATCC GTATCC(6/5)
BclI 2120 TGATCA T^GATCA
BfrI 1149 CTTAAG C^TTAAG
BglII 456 AGATCT A^GATCT
BsaAI 1947 CACGTG [CT]AC^GT[GA]
BsaBI 2142 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1987 GAGACC GGTCTC(1/5)
BseRI 2557 GAGGAG GAGGAG(10/8)
BsgI 363 CTGCAC GTGCAG(16/14)
BsmI 746 GCATTC GAATGC(1/-1)
Bsp1407I 2706 TGTACA T^GTACA
BspDI 1771 ATCGAT AT^CGAT
BspHI 2117 TCATGA T^CATGA
BspLU11I 1571 ACATGT A^CATGT
BspMI 1403 ACCTGC ACCTGC(4/8)
BsrGI 2706 TGTACA T^GTACA
BstBI 1375 TTCGAA TT^CGAA
BstXI 2537 CCATTATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1771 ATCGAT AT^CGAT
DraIII 75 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2635 TGGCCA [CT]^GGCC[GA]
Eco47III 552 AGCGCT AGC^GCT
EcoT14I 1430 CCTTGG C^C[AT][AT]GG
FauI 2383 GCGGG CCCGC(4/6)
FbaI 2120 TGATCA T^GATCA
HaeII 552 AGCGCT [GA]GCGC^[CT]
HpaI 2410 GTTAAC GTT^AAC
MaeII 1948 ACGT A^CGT
MamI 2142 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2635 TGGCCA TGG^CCA
MscI 2635 TGGCCA TGG^CCA
NspI 1571 ACATGT [GA]CATG^[CT]
NspV 1375 TTCGAA TT^CGAA
PmaCI 1947 CACGTG CAC^GTG
PmlI 1947 CACGTG CAC^GTG
PpuMI 699 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2117 TCATGA T^CATGA
ScaI 1302 AGTACT AGT^ACT
SfuI 1375 TTCGAA TT^CGAA
SspBI 2706 TGTACA T^GTACA
StyI 1430 CCTTGG C^C[AT][AT]GG
XbaI 1362 TCTAGA T^CTAGA
XcmI 257 CCATCTCAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1881 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 132 GGTACC G^GTACC
974 GGTACC
AciI 203 CCGC CCGC(-3/-1)
2383 GCGG
AflIII 1571 ACATGT A^C[GA][CT]GT
1948 ACGTGT
Asp718I 132 GGTACC G^GTACC
974 GGTACC
AspHI 81 GTGCAC G[AT]GC[AT]^C
1072 GAGCAC
BbsI 2338 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BpuAI 2338 GAAGAC GAAGAC(2/6)
1741 GTCTTC
BsiHKAI 81 GTGCAC G[AT]GC[AT]^C
1072 GAGCAC
CfoI 553 GCGC GCG^C
1926 GCGC
DraII 35 GGGGCCT [GA]G^G[ACGT]CC[CT]
699 GGGTCCC
EcoO109I 35 GGGGCCT [GA]G^G[ACGT]CC[CT]
699 GGGTCCC
EcoRV 574 GATATC GAT^ATC
2187 GATATC
HhaI 553 GCGC GCG^C
1926 GCGC
HinP1I 553 GCGC G^CGC
1926 GCGC
HincII 123 GTTGAC GT[CT]^[GA]AC
2410 GTTAAC
HindII 123 GTTGAC GT[CT]^[GA]AC
2410 GTTAAC
HphI 1498 TCACC GGTGA(8/7)
1686 TCACC
KpnI 132 GGTACC GGTAC^C
974 GGTACC
MfeI 656 CAATTG C^AATTG
2405 CAATTG
MslI 156 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2044 CACTATCATG
MunI 656 CAATTG C^AATTG
2405 CAATTG
PleI 126 GACTC GAGTC(4/5)
1708 GAGTC
PstI 108 CTGCAG CTGCA^G
1405 CTGCAG
SfcI 108 CTGCAG C^T[GA][CT]AG
1405 CTGCAG
SspI 902 AATATT AAT^ATT
1369 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanI BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII