Restriction map for
pFN21AE6641
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 677 AAATTT [GA]^AATT[CT]
AcyI 135 GACGCC G[GA]^CG[CT]C
AhdI 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 73 AGCT AG^CT
AlwI 286 GGATC GGATC(4/5)
AlwNI 386 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 323 AGCGCT AGC^GCT
ApoI 677 AAATTT [GA]^AATT[CT]
AspEI 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 599 GGACC G^G[AT]CC
BanII 72 GAGCTC G[GA]GC[CT]^C
BbsI 497 GAAGAC GAAGAC(2/6)
BcgI 438 GCACAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 380 GTATCC GTATCC(6/5)
BcnI 417 CCCGG CC^[GC]GG
BfaI 493 CTAG C^TAG
BpuAI 497 GAAGAC GAAGAC(2/6)
BsaHI 135 GACGCC G[GA]^CG[CT]C
BsaI 539 GAGACC GGTCTC(1/5)
BseRI 741 CTCCTC GAGGAG(10/8)
BsmBI 133 GAGACG CGTCTC(1/5)
BsmFI 275 GTCCC GGGAC(10/14)
BsmI 184 GCATTC GAATGC(1/-1)
BspDI 155 ATCGAT AT^CGAT
BstAPI 341 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 330 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 324 GCGC GCG^C
ClaI 155 ATCGAT AT^CGAT
DraII 704 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 323 AGCGCT AGC^GCT
EcoNI 213 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 704 AGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 323 AGCGCT [GA]GCGC^[CT]
HapII 418 CCGG C^CGG
HgaI 135 GACGC GACGC(5/10)
HhaI 324 GCGC GCG^C
Hin1I 135 GACGCC G[GA]^CG[CT]C
HinP1I 324 GCGC G^CGC
HpaII 418 CCGG C^CGG
KspI 53 CCGCGG CCGC^GG
MaeI 493 CTAG C^TAG
MboII 497 GAAGA GAAGA(8/7)
MseI 403 TTAA T^TAA
MspI 418 CCGG C^CGG
NciI 417 CCCGG CC^[GC]GG
PleI 755 GACTC GAGTC(4/5)
SacI 72 GAGCTC GAGCT^C
SacII 53 CCGCGG CCGC^GG
StuI 221 AGGCCT AGG^CCT
Tru9I 403 TTAA T^TAA
Tsp45I 516 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BsmAI 133 GAGAC GTCTC(1/5)
539 GAGAC
BstNI 139 CCTGG CC^[AT]GG
213 CCTGG
DdeI 434 CTCAG C^T[ACGT]AG
509 CTAAG
DsaI 53 CCGCGG C^C[GA][CT]GG
453 CCACGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
50 TGGCCG
EcoRII 139 CCTGG ^CC[AT]GG
213 CCTGG
EcoT14I 174 CCAAGG C^C[AT][AT]GG
282 CCAAGG
MaeIII 388 GTTAC ^GT[ACGT]AC
516 GTCAC
MslI 331 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
507 CACTAAGATG
MspA1I 53 CCGCGG C[AC]G^C[GT]G
360 CCGCTG
MvaI 139 CCTGG CC^[AT]GG
213 CCTGG
NspI 167 GCATGT [GA]CATG^[CT]
341 GCATGT
PflMI 174 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
471 CCATGACTTGG
StyI 174 CCAAGG C^C[AT][AT]GG
282 CCAAGG
TaqI 156 TCGA T^CGA
447 TCGA
TfiI 25 GATTC G^A[AT]TC
489 GAATC
Tsp509I 678 AATT ^AATT
774 AATT
TspRI 237 CAGTG CA[GC]TG[ACGT][ACGT]^
617 CAGTG
TthHB8I 156 TCGA T^CGA
447 TCGA
Van91I 174 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
471 CCATGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AfaI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstUI
Cac8I Cfr13I DpnII FauI Fnu4HI
FokI HaeIII HinfI ItaI MboI
MnlI MvnI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI