Restriction map for
pFN21AE2253
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FokI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AlwI 658 GATCC GGATC(4/5)
Asp700I 4 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 754 GAGCAC G[AT]GC[AT]^C
BclI 684 TGATCA T^GATCA
BcnI 661 CCGGG CC^[GC]GG
BsgI 761 CTGCAC GTGCAG(16/14)
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
BspHI 741 TCATGA T^CATGA
BstNI 412 CCAGG CC^[AT]GG
BstYI 657 AGATCC [GA]^GATC[CT]
Cac8I 535 GCAAGC GC[ACGT]^[ACGT]GC
EcoRII 412 CCAGG ^CC[AT]GG
EcoT22I 434 ATGCAT ATGCA^T
FbaI 684 TGATCA T^GATCA
HincII 225 GTCAAC GT[CT]^[GA]AC
HindII 225 GTCAAC GT[CT]^[GA]AC
HindIII 715 AAGCTT A^AGCTT
MaeII 311 ACGT A^CGT
MflI 657 AGATCC [GA]^GATC[CT]
MvaI 412 CCAGG CC^[AT]GG
MwoI 50 GCAGTAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 661 CCGGG CC^[GC]GG
NlaIII 742 CATG CATG^
NsiI 434 ATGCAT ATGCA^T
RcaI 741 TCATGA T^CATGA
SexAI 411 ACCAGGT A^CC[AT]GGT
SmlI 503 CTCAAG C^T[CT][GA]AG
TfiI 136 GAATC G^A[AT]TC
XhoII 657 AGATCC [GA]^GATC[CT]
XmnI 4 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 455 GTAC GT^AC
636 GTAC
BbvI 47 GCAGC GCAGC(8/12)
76 GCAGC
BmyI 663 GGGCAC G[AGT]GC[ACT]^C
754 GAGCAC
BsiYI 163 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
670 CCAGAAAATGG
BslI 163 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
670 CCAGAAAATGG
BsmAI 216 GTCTC GTCTC(1/5)
501 GTCTC
Bsp1286I 663 GGGCAC G[AGT]GC[ACT]^C
754 GAGCAC
BsrFI 554 GCCGGT [GA]^CCGG[CT]
579 GCCGGT
BssKI 412 CCAGG ^CC[ACGT]GG
661 CCGGG
Cfr10I 554 GCCGGT [GA]^CCGG[CT]
579 GCCGGT
DpnII 658 GATC ^GATC
685 GATC
Fnu4HI 47 GCAGC GC^[ACGT]GC
76 GCAGC
ItaI 47 GCAGC GC^[ACGT]GC
76 GCAGC
MboI 658 GATC ^GATC
685 GATC
MboII 288 GAAGA GAAGA(8/7)
30 TCTTC
NdeII 658 GATC ^GATC
685 GATC
PflMI 163 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
670 CCAGAAAATGG
PleI 295 GACTC GAGTC(4/5)
745 GAGTC
RsaI 455 GTAC GT^AC
636 GTAC
Sau3AI 658 GATC ^GATC
685 GATC
ScrFI 412 CCAGG CC^[ACGT]GG
661 CCGGG
SfaNI 433 GATGC GCATC(5/9)
436 GCATC
TseI 47 GCAGC G^C[AT]GC
76 GCAGC
Tsp45I 16 GTGAC ^GT[GC]AC
157 GTCAC
TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^
516 CACTG
Van91I 163 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
670 CCAGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BsrI DdeI HapII HinfI
HpaII HphI MaeIII MnlI MseI
MspI Tru9I Tsp509I