Restriction map for
pFN21AE6388
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MluI MseI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfcI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tru9I Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 164 TCCGGA T^CCGGA
AciI 407 CCGC CCGC(-3/-1)
AclI 337 AACGTT AA^CGTT
AcsI 660 GAATTC [GA]^AATT[CT]
AfaI 930 GTAC GT^AC
AlwNI 626 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 660 GAATTC [GA]^AATT[CT]
BbsI 458 GTCTTC GAAGAC(2/6)
BpuAI 458 GTCTTC GAAGAC(2/6)
BsaWI 164 TCCGGA [AT]^CCGG[AT]
BseAI 164 TCCGGA T^CCGGA
BsgI 762 GTGCAG GTGCAG(16/14)
BspEI 164 TCCGGA T^CCGGA
BsrDI 201 CATTGC GCAATG(2/0)
BstF5I 148 GGATG GGATG(2/0)
BstXI 518 CCATTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 605 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 883 CTCTTC CTCTTC(1/4)
EcoO109I 605 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 660 GAATTC G^AATTC
FokI 148 GGATG GGATG(9/13)
Ksp632I 883 CTCTTC CTCTTC(1/4)
MaeIII 715 GTCAC ^GT[ACGT]AC
MfeI 9 CAATTG C^AATTG
MroI 164 TCCGGA T^CCGGA
MspA1I 889 CAGCTG C[AC]G^C[GT]G
MunI 9 CAATTG C^AATTG
PaeR7I 1099 CTCGAG C^TCGAG
PpuMI 605 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 337 AACGTT AA^CGTT
PvuII 889 CAGCTG CAG^CTG
RsaI 930 GTAC GT^AC
SexAI 445 ACCTGGT A^CC[AT]GGT
SfiI 1067 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 318 CCCGGG CCC^GGG
SspI 796 AATATT AAT^ATT
TfiI 468 GAATC G^A[AT]TC
Tsp45I 715 GTCAC ^GT[GC]AC
XhoI 1099 CTCGAG C^TCGAG
XmaCI 318 CCCGGG C^CCGGG
XmaI 318 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 318 CCCGGG C^[CT]CG[GA]G
1099 CTCGAG
BanI 325 GGCACC G^G[CT][GA]CC
1051 GGTGCC
BcnI 318 CCCGG CC^[GC]GG
319 CCGGG
BglI 317 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1068 GCCACCAGGGC
BlpI 23 GCTCAGC GC^T[ACGT]AGC
838 GCTGAGC
Bpu1102I 23 GCTCAGC GC^T[ACGT]AGC
838 GCTGAGC
BsaBI 364 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
804 GATGTGCATC
BsaI 189 GAGACC GGTCTC(1/5)
1018 GAGACC
BsmAI 189 GAGAC GTCTC(1/5)
1018 GAGAC
BsmFI 66 GGGAC GGGAC(10/14)
210 GGGAC
BsoBI 318 CCCGGG C^[CT]CG[GA]G
1099 CTCGAG
Cac8I 28 GCCTGC GC[ACGT]^[ACGT]GC
729 GCTGGC
CelII 23 GCTCAGC GC^T[ACGT]AGC
838 GCTGAGC
DsaI 155 CCGTGG C^C[GA][CT]GG
401 CCGTGG
HapII 165 CCGG C^CGG
319 CCGG
HpaII 165 CCGG C^CGG
319 CCGG
MaeII 338 ACGT A^CGT
928 ACGT
MamI 364 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
804 GATGTGCATC
MspI 165 CCGG C^CGG
319 CCGG
NciI 318 CCCGG CC^[GC]GG
319 CCGGG
PleI 881 GACTC GAGTC(4/5)
456 GAGTC
SacI 91 GAGCTC GAGCT^C
788 GAGCTC
SmlI 580 CTCAAG C^T[CT][GA]AG
1099 CTCGAG
TaqI 1003 TCGA T^CGA
1100 TCGA
TthHB8I 1003 TCGA T^CGA
1100 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AluI AlwI AspHI AvaII BalI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MboI
MboII MflI MluNI MnlI MscI
MslI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I TspRI XcmI XhoII