Restriction map for
pFN21AE3014
##################################
# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 31 CGCG CG^CG
AcsI 251 AAATTT [GA]^AATT[CT]
AfaI 279 GTAC GT^AC
AlwI 261 GGATC GGATC(4/5)
AlwNI 127 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 251 AAATTT [GA]^AATT[CT]
AspHI 3 GAGCTC G[AT]GC[AT]^C
BanII 3 GAGCTC G[GA]GC[CT]^C
BbsI 141 GAAGAC GAAGAC(2/6)
BclI 188 TGATCA T^GATCA
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
BpmI 181 CTGGAG CTGGAG(16/14)
BpuAI 141 GAAGAC GAAGAC(2/6)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsiYI 8 CCGCAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 8 CCGCAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 278 TGTACA T^GTACA
BspMI 122 ACCTGC ACCTGC(4/8)
BsrGI 278 TGTACA T^GTACA
BsrI 76 CCAGT ACTGG(1/-1)
BstAPI 267 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 31 CGCG CG^CG
BstYI 261 GGATCT [GA]^GATC[CT]
Cac8I 267 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 32 GCGC GCG^C
FbaI 188 TGATCA T^GATCA
HaeIII 195 GGCC GG^CC
HapII 197 CCGG C^CGG
HhaI 32 GCGC GCG^C
HinP1I 32 GCGC G^CGC
HpaII 197 CCGG C^CGG
MflI 261 GGATCT [GA]^GATC[CT]
MseI 293 TTAA T^TAA
MspI 197 CCGG C^CGG
MvnI 31 CGCG CG^CG
PstI 124 CTGCAG CTGCA^G
RsaI 279 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
SfaNI 48 GATGC GCATC(5/9)
SfcI 124 CTGCAG C^T[GA][CT]AG
SspBI 278 TGTACA T^GTACA
Tru9I 293 TTAA T^TAA
Tsp509I 252 AATT ^AATT
XhoII 261 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BbvI 10 GCAGC GCAGC(8/12)
66 GCTGC
BsaJI 152 CCCAGG C^C[ACGT][ACGT]GG
163 CCCAGG
DpnII 189 GATC ^GATC
262 GATC
DraII 16 AGGTCCC [GA]G^G[ACGT]CC[CT]
119 GGGACCT
EcoO109I 16 AGGTCCC [GA]G^G[ACGT]CC[CT]
119 GGGACCT
Fnu4HI 10 GCAGC GC^[ACGT]GC
66 GCTGC
ItaI 10 GCAGC GC^[ACGT]GC
66 GCTGC
MboI 189 GATC ^GATC
262 GATC
NdeII 189 GATC ^GATC
262 GATC
PpuMI 16 AGGTCCC [GA]G^G[AT]CC[CT]
119 GGGACCT
Sau3AI 189 GATC ^GATC
262 GATC
TseI 10 GCAGC G^C[AT]GC
66 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI AvaII BsmFI BssKI
BstNI Cfr13I EcoRII FauI HphI
MboII MnlI MvaI MwoI NlaIV
Sau96I ScrFI TspRI