Restriction map for
pFN21AE2050
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI HphI KasI
KpnI Ksp632I KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI StuI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 1745 AACGTT AA^CGTT
AgeI 56 ACCGGT A^CCGGT
Alw44I 551 GTGCAC G^TGCAC
ApaLI 551 GTGCAC G^TGCAC
AspHI 551 GTGCAC G[AT]GC[AT]^C
AvaI 1003 CCCGAG C^[CT]CG[GA]G
BanII 1952 GGGCTC G[GA]GC[CT]^C
BcnI 1758 CCGGG CC^[GC]GG
BfaI 787 CTAG C^TAG
BglI 65 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 383 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 383 GCTTAGC GC^T[ACGT]AGC
BsaBI 1096 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 56 ACCGGT [AT]^CCGG[AT]
BsiHKAI 551 GTGCAC G[AT]GC[AT]^C
BsiYI 1876 CCAAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1876 CCAAGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1536 GTCTC GTCTC(1/5)
BsoBI 1003 CCCGAG C^[CT]CG[GA]G
Bsp1407I 944 TGTACA T^GTACA
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrFI 56 ACCGGT [GA]^CCGG[CT]
BsrGI 944 TGTACA T^GTACA
BssHII 84 GCGCGC G^CGCGC
BstAPI 457 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1720 CCAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 383 GCTTAGC GC^T[ACGT]AGC
Cfr10I 56 ACCGGT [GA]^CCGG[CT]
DraII 1801 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 826 GACCAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1133 CCATGG C^C[GA][CT]GG
EcoO109I 1801 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 17 GCGGG CCCGC(4/6)
HincII 1622 GTCAAC GT[CT]^[GA]AC
HindII 1622 GTCAAC GT[CT]^[GA]AC
HinfI 841 GACTC G^A[ACGT]TC
MaeI 787 CTAG C^TAG
MamI 1096 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 477 CAATTG C^AATTG
MslI 213 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 477 CAATTG C^AATTG
NciI 1758 CCGGG CC^[GC]GG
NcoI 1133 CCATGG C^CATGG
PflMI 1876 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 56 ACCGGT A^CCGGT
PleI 841 GACTC GAGTC(4/5)
PpuMI 1801 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1745 AACGTT AA^CGTT
SbfI 1945 CCTGCAGG CCTGCA^GG
Sse8387I 1945 CCTGCAGG CCTGCA^GG
SspBI 944 TGTACA T^GTACA
TaqI 720 TCGA T^CGA
Tsp45I 1403 GTGAC ^GT[GC]AC
TthHB8I 720 TCGA T^CGA
Van91I 1876 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 83 CGCG CG^CG
85 CGCG
AlwNI 29 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1898 CAGTATCTG
AvaII 825 GGACC G^G[AT]CC
1802 GGACC
BalI 63 TGGCCA TGG^CCA
674 TGGCCA
BglII 399 AGATCT A^GATCT
609 AGATCT
BmyI 551 GTGCAC G[AGT]GC[ACT]^C
1952 GGGCTC
BpmI 1955 CTCCAG CTGGAG(16/14)
1613 CTGGAG
BsmI 285 GAATGC GAATGC(1/-1)
379 GAATGC
Bsp1286I 551 GTGCAC G[AGT]GC[ACT]^C
1952 GGGCTC
BspMI 33 ACCTGC ACCTGC(4/8)
1944 ACCTGC
BstF5I 1102 CATCC GGATG(2/0)
1839 GGATG
BstUI 83 CGCG CG^CG
85 CGCG
EaeI 63 TGGCCA [CT]^GGCC[GA]
674 TGGCCA
EcoT14I 564 CCAAGG C^C[AT][AT]GG
1133 CCATGG
FokI 1102 CATCC GGATG(9/13)
1839 GGATG
HapII 57 CCGG C^CGG
1758 CCGG
HpaII 57 CCGG C^CGG
1758 CCGG
MaeII 131 ACGT A^CGT
1746 ACGT
MluNI 63 TGGCCA TGG^CCA
674 TGGCCA
MscI 63 TGGCCA TGG^CCA
674 TGGCCA
MspI 57 CCGG C^CGG
1758 CCGG
MvnI 83 CGCG CG^CG
85 CGCG
NspI 547 GCATGT [GA]CATG^[CT]
1174 GCATGT
SmlI 91 CTCAAG C^T[CT][GA]AG
1154 CTCAAG
SspI 1162 AATATT AAT^ATT
1182 AATATT
StyI 564 CCAAGG C^C[AT][AT]GG
1133 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BpuAI BsaJI
BseRI BsmFI BsrI BssKI BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HhaI
HinP1I HindIII ItaI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI XhoII