Restriction map for
pFN21AE4129
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
AlwI AlwNI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BglI BglII BlpI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
BstYI CelII CfoI Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 274 GTATAC GT^[AC][GT]AC
AflII 838 CTTAAG C^TTAAG
Alw44I 336 GTGCAC G^TGCAC
ApaLI 336 GTGCAC G^TGCAC
AspHI 336 GTGCAC G[AT]GC[AT]^C
AvaI 971 CCCGGG C^[CT]CG[GA]G
AvaII 18 GGACC G^G[AT]CC
AvrII 1404 CCTAGG C^CTAGG
BbsI 104 GTCTTC GAAGAC(2/6)
BfrI 838 CTTAAG C^TTAAG
BlnI 1404 CCTAGG C^CTAGG
BpuAI 104 GTCTTC GAAGAC(2/6)
BsaAI 686 TACGTA [CT]AC^GT[GA]
BsaI 111 GAGACC GGTCTC(1/5)
BseRI 10 GAGGAG GAGGAG(10/8)
BsgI 242 CTGCAC GTGCAG(16/14)
BsiHKAI 336 GTGCAC G[AT]GC[AT]^C
BsmAI 111 GAGAC GTCTC(1/5)
BsmFI 755 GTCCC GGGAC(10/14)
BsoBI 971 CCCGGG C^[CT]CG[GA]G
BsrDI 911 GCAATG GCAATG(2/0)
Bst1107I 274 GTATAC GTA^TAC
BstAPI 1507 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 95 CCTGG CC^[AT]GG
BstZ17I 274 GTATAC GTA^TAC
DpnII 1375 GATC ^GATC
DrdI 746 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 758 CCACGG C^C[GA][CT]GG
EcoNI 1473 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 154 GAATTC G^AATTC
EcoRII 95 CCTGG ^CC[AT]GG
EcoT14I 1404 CCTAGG C^C[AT][AT]GG
HapII 972 CCGG C^CGG
HincII 900 GTTGAC GT[CT]^[GA]AC
HindII 900 GTTGAC GT[CT]^[GA]AC
HpaII 972 CCGG C^CGG
MaeII 687 ACGT A^CGT
MboI 1375 GATC ^GATC
MslI 1331 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 972 CCGG C^CGG
MvaI 95 CCTGG CC^[AT]GG
NdeII 1375 GATC ^GATC
NlaIV 61 GGCTCC GG[ACGT]^[ACGT]CC
NspI 681 GCATGT [GA]CATG^[CT]
PacI 484 TTAATTAA TTAAT^TAA
PflMI 1075 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1496 CTGCAG CTGCA^G
Sau3AI 1375 GATC ^GATC
SmaI 971 CCCGGG CCC^GGG
SnaBI 686 TACGTA TAC^GTA
SspI 796 AATATT AAT^ATT
StuI 92 AGGCCT AGG^CCT
StyI 1404 CCTAGG C^C[AT][AT]GG
TaqI 1347 TCGA T^CGA
TthHB8I 1347 TCGA T^CGA
Van91I 1075 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 971 CCCGGG C^CCGGG
XmaI 971 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AocI 463 CCTAAGG CC^T[ACGT]AGG
811 CCTGAGG
AseI 483 ATTAAT AT^TAAT
487 ATTAAT
AsnI 483 ATTAAT AT^TAAT
487 ATTAAT
Asp700I 1255 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1504 GAAGCAGTTC
BcnI 971 CCCGG CC^[GC]GG
972 CCGGG
Bsu36I 463 CCTAAGG CC^T[ACGT]AGG
811 CCTGAGG
Cfr13I 18 GGACC G^G[ACGT]CC
98 GGGCC
Eco81I 463 CCTAAGG CC^T[ACGT]AGG
811 CCTGAGG
NciI 971 CCCGG CC^[GC]GG
972 CCGGG
PshBI 483 ATTAAT AT^TAAT
487 ATTAAT
Sau96I 18 GGACC G^G[ACGT]CC
98 GGGCC
SfcI 1370 CTACAG C^T[GA][CT]AG
1496 CTGCAG
XmnI 1255 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1504 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanII
BbvI BciVI BfaI BmyI BsaJI
BsiYI BslI BsmI Bsp1286I BsrI
BssKI BstF5I Cac8I DdeI DraI
EarI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MspA1I MwoI
NlaIII PleI PvuII RsaI ScrFI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI