Restriction map for
pFN21AE3061
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
ClaI CpoI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HphI
ItaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
MwoI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TseI Tsp45I Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 572 GTAC GT^AC
AhdI 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 452 AGCT AG^CT
Asp700I 322 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 428 GAGCAC G[AT]GC[AT]^C
AvaII 4 GGTCC G^G[AT]CC
BamHI 243 GGATCC G^GATCC
BanI 126 GGTGCC G^G[CT][GA]CC
BlpI 453 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 453 GCTAAGC GC^T[ACGT]AGC
BsiHKAI 428 GAGCAC G[AT]GC[AT]^C
BspMI 162 GCAGGT ACCTGC(4/8)
BsrFI 395 GCCGGC [GA]^CCGG[CT]
CelII 453 GCTAAGC GC^T[ACGT]AGC
Cfr10I 395 GCCGGC [GA]^CCGG[CT]
DraII 3 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 3 GGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 396 CCGG C^CGG
HincII 622 GTTAAC GT[CT]^[GA]AC
HindII 622 GTTAAC GT[CT]^[GA]AC
HpaI 622 GTTAAC GTT^AAC
HpaII 396 CCGG C^CGG
MaeIII 68 GTTAC ^GT[ACGT]AC
MspI 396 CCGG C^CGG
NaeI 395 GCCGGC GCC^GGC
NgoMIV 395 GCCGGC G^CCGGC
PflMI 188 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 572 GTAC GT^AC
SfaNI 640 GCATC GCATC(5/9)
SmlI 145 CTTGAG C^T[CT][GA]AG
TaqI 270 TCGA T^CGA
TthHB8I 270 TCGA T^CGA
Van91I 188 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 403 TCTAGA T^CTAGA
XcmI 325 CCAATTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 322 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BfaI 8 CTAG C^TAG
404 CTAG
BmyI 127 GTGCCC G[AGT]GC[ACT]^C
428 GAGCAC
BsiYI 188 CCACAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
312 CCCCAAACTGG
BslI 188 CCACAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
312 CCCCAAACTGG
Bsp1286I 127 GTGCCC G[AGT]GC[ACT]^C
428 GAGCAC
BsrI 318 ACTGG ACTGG(1/-1)
256 CCAGT
BssKI 158 CCTGG ^CC[ACGT]GG
218 CCTGG
BstNI 158 CCTGG CC^[AT]GG
218 CCTGG
BstYI 243 GGATCC [GA]^GATC[CT]
503 AGATCC
Cac8I 395 GCCGGC GC[ACGT]^[ACGT]GC
539 GCTGGC
Cfr13I 4 GGTCC G^G[ACGT]CC
338 GGCCC
DdeI 283 CTAAG C^T[ACGT]AG
454 CTAAG
DraI 177 TTTAAA TTT^AAA
204 TTTAAA
EarI 91 GAAGAG CTCTTC(1/4)
112 GAAGAG
EcoRII 158 CCTGG ^CC[AT]GG
218 CCTGG
HaeIII 338 GGCC GG^CC
348 GGCC
Ksp632I 91 GAAGAG CTCTTC(1/4)
112 GAAGAG
MaeI 8 CTAG C^TAG
404 CTAG
MflI 243 GGATCC [GA]^GATC[CT]
503 AGATCC
MvaI 158 CCTGG CC^[AT]GG
218 CCTGG
Sau96I 4 GGTCC G^G[ACGT]CC
338 GGCCC
ScrFI 158 CCTGG CC^[ACGT]GG
218 CCTGG
SspI 61 AATATT AAT^ATT
182 AATATT
TfiI 37 GAATC G^A[AT]TC
100 GATTC
XhoII 243 GGATCC [GA]^GATC[CT]
503 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AlwI BsmFI DpnII HinfI MboI
MboII MnlI MseI NdeII NlaIII
NlaIV PleI Sau3AI Tru9I Tsp509I
TspRI