Restriction map for
pFN21AE1849
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BsaBI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstYI BstZ17I Cfr10I
ClaI CpoI DpnII DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboI MflI MluI MluNI
MscI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 892 CGCG CG^CG
AccIII 842 TCCGGA T^CCGGA
AcsI 675 GAATTT [GA]^AATT[CT]
AcyI 505 GACGCC G[GA]^CG[CT]C
AocI 294 CCTCAGG CC^T[ACGT]AGG
ApaI 556 GGGCCC GGGCC^C
ApoI 675 GAATTT [GA]^AATT[CT]
AvaI 1077 CCCGAG C^[CT]CG[GA]G
BanII 556 GGGCCC G[GA]GC[CT]^C
BbrPI 277 CACGTG CAC^GTG
BciVI 113 GGATAC GTATCC(6/5)
BglI 422 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 556 GGGCCC G[AGT]GC[ACT]^C
BpmI 354 CTCCAG CTGGAG(16/14)
BsaAI 277 CACGTG [CT]AC^GT[GA]
BsaHI 505 GACGCC G[GA]^CG[CT]C
BsaI 793 GAGACC GGTCTC(1/5)
BseAI 842 TCCGGA T^CCGGA
BseRI 904 GAGGAG GAGGAG(10/8)
BsoBI 1077 CCCGAG C^[CT]CG[GA]G
Bsp1286I 556 GGGCCC G[AGT]GC[ACT]^C
BspEI 842 TCCGGA T^CCGGA
BsrDI 123 CATTGC GCAATG(2/0)
BsrI 896 ACTGG ACTGG(1/-1)
BssSI 638 CACGAG CACGAG(-5/-1)
BstAPI 831 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 85 GGTAACC G^GT[ACGT]ACC
BstPI 85 GGTAACC G^GT[ACGT]ACC
BstUI 892 CGCG CG^CG
Bsu36I 294 CCTCAGG CC^T[ACGT]AGG
CfoI 1010 GCGC GCG^C
DraIII 853 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 505 GACGCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1060 CGGCCA [CT]^GGCC[GA]
Eco81I 294 CCTCAGG CC^T[ACGT]AGG
EcoO65I 85 GGTAACC G^GT[ACGT]ACC
EcoT22I 991 ATGCAT ATGCA^T
HhaI 1010 GCGC GCG^C
Hin1I 505 GACGCC G[GA]^CG[CT]C
HinP1I 1010 GCGC G^CGC
HindIII 362 AAGCTT A^AGCTT
MaeII 278 ACGT A^CGT
MfeI 144 CAATTG C^AATTG
MroI 842 TCCGGA T^CCGGA
MspA1I 539 CCGCTG C[AC]G^C[GT]G
MunI 144 CAATTG C^AATTG
MvnI 892 CGCG CG^CG
NsiI 991 ATGCAT ATGCA^T
PflMI 527 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 277 CACGTG CAC^GTG
PmlI 277 CACGTG CAC^GTG
PpuMI 570 AGGACCC [GA]G^G[AT]CC[CT]
PstI 829 CTGCAG CTGCA^G
SexAI 854 ACCAGGT A^CC[AT]GGT
SfcI 829 CTGCAG C^T[GA][CT]AG
TaqI 717 TCGA T^CGA
TfiI 300 GATTC G^A[AT]TC
TthHB8I 717 TCGA T^CGA
Van91I 527 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 864 CCAAGTTGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 225 GTAC GT^AC
390 GTAC
BlpI 541 GCTGAGC GC^T[ACGT]AGC
607 GCTGAGC
Bpu1102I 541 GCTGAGC GC^T[ACGT]AGC
607 GCTGAGC
BsaWI 842 TCCGGA [AT]^CCGG[AT]
930 ACCGGA
BstXI 424 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAACGAGCTGG
CelII 541 GCTGAGC GC^T[ACGT]AGC
607 GCTGAGC
EcoT14I 109 CCATGG C^C[AT][AT]GG
1041 CCATGG
HgaI 505 GACGC GACGC(5/10)
834 GACGC
HphI 263 TCACC GGTGA(8/7)
996 TCACC
MslI 91 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
997 CACCCTGATG
NcoI 109 CCATGG C^CATGG
1041 CCATGG
RsaI 225 GTAC GT^AC
390 GTAC
SfaNI 31 GATGC GCATC(5/9)
993 GCATC
StyI 109 CCATGG C^C[AT][AT]GG
1041 CCATGG
Tsp45I 479 GTCAC ^GT[GC]AC
485 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbsI
BbvI BcnI BpuAI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboII MnlI MseI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI