Restriction map for
pFN21AE5577
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII BamHI BbeI BciVI BclI
BfrI BglII BlpI Bpu1102I BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MflI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 928 GTCTAC GT^[AC][GT]AC
AccII 1260 CGCG CG^CG
AciI 1281 CCGC CCGC(-3/-1)
AcyI 405 GACGCC G[GA]^CG[CT]C
AfaI 527 GTAC GT^AC
AflIII 608 ACACGT A^C[GA][CT]GT
AlwI 399 GGATC GGATC(4/5)
AseI 298 ATTAAT AT^TAAT
AsnI 298 ATTAAT AT^TAAT
AspHI 646 GAGCTC G[AT]GC[AT]^C
AvaII 1180 GGACC G^G[AT]CC
AvrII 1030 CCTAGG C^CTAGG
BbrPI 1000 CACGTG CAC^GTG
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
BcgI 825 CGAGAATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1153 CCGGG CC^[GC]GG
BfaI 1031 CTAG C^TAG
BlnI 1030 CCTAGG C^CTAGG
BsaAI 1000 CACGTG [CT]AC^GT[GA]
BsaBI 394 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 405 GACGCC G[GA]^CG[CT]C
BsaWI 412 TCCGGT [AT]^CCGG[AT]
BsiHKAI 646 GAGCTC G[AT]GC[AT]^C
BsmBI 611 CGTCTC CGTCTC(1/5)
BspHI 881 TCATGA T^CATGA
BstEII 471 GGTGACC G^GT[ACGT]ACC
BstPI 471 GGTGACC G^GT[ACGT]ACC
BstUI 1260 CGCG CG^CG
DrdI 121 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 471 GGTGACC G^GT[ACGT]ACC
Hin1I 405 GACGCC G[GA]^CG[CT]C
MaeI 1031 CTAG C^TAG
MamI 394 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 90 CAATTG C^AATTG
MspA1I 247 CAGCTG C[AC]G^C[GT]G
MunI 90 CAATTG C^AATTG
MvnI 1260 CGCG CG^CG
NciI 1153 CCGGG CC^[GC]GG
NspI 566 GCATGC [GA]CATG^[CT]
PmaCI 1000 CACGTG CAC^GTG
PmlI 1000 CACGTG CAC^GTG
PpuMI 1179 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 298 ATTAAT AT^TAAT
PvuII 247 CAGCTG CAG^CTG
RcaI 881 TCATGA T^CATGA
RsaI 527 GTAC GT^AC
SacI 646 GAGCTC GAGCT^C
SfcI 177 CTACAG C^T[GA][CT]AG
SphI 566 GCATGC GCATG^C
StuI 155 AGGCCT AGG^CCT
TaqI 444 TCGA T^CGA
Tsp45I 472 GTGAC ^GT[GC]AC
TthHB8I 444 TCGA T^CGA
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# TWO CUT ENZYMES ( 35 enzymes) #
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AlwNI 247 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
477 CAGCATCTG
BalI 722 TGGCCA TGG^CCA
1004 TGGCCA
BanI 765 GGTGCC G^G[CT][GA]CC
910 GGCACC
BanII 646 GAGCTC G[GA]GC[CT]^C
1206 GGGCTC
BglI 15 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1029 GCCTAGGTGGC
BpmI 244 CTCCAG CTGGAG(16/14)
970 CTGGAG
BseRI 34 CTCCTC GAGGAG(10/8)
1305 CTCCTC
BsrI 770 CCAGT ACTGG(1/-1)
905 CCAGT
BstXI 389 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCACCTGCCTGG
CfoI 732 GCGC GCG^C
1090 GCGC
DraII 1109 AGGGCCT [GA]G^G[ACGT]CC[CT]
1179 GGGACCC
DsaI 389 CCATGG C^C[GA][CT]GG
683 CCATGG
EaeI 722 TGGCCA [CT]^GGCC[GA]
1004 TGGCCA
EcoO109I 1109 AGGGCCT [GA]G^G[ACGT]CC[CT]
1179 GGGACCC
HapII 413 CCGG C^CGG
1153 CCGG
HgaI 76 GACGC GACGC(5/10)
405 GACGC
HhaI 732 GCGC GCG^C
1090 GCGC
HinP1I 732 GCGC G^CGC
1090 GCGC
HincII 118 GTTGAC GT[CT]^[GA]AC
196 GTCAAC
HindII 118 GTTGAC GT[CT]^[GA]AC
196 GTCAAC
HinfI 385 GAATC G^A[ACGT]TC
828 GAATC
HpaII 413 CCGG C^CGG
1153 CCGG
MaeIII 472 GTGAC ^GT[ACGT]AC
485 GTAAC
MluNI 722 TGGCCA TGG^CCA
1004 TGGCCA
MscI 722 TGGCCA TGG^CCA
1004 TGGCCA
MseI 299 TTAA T^TAA
449 TTAA
MslI 260 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
390 CATGGATGTG
MspI 413 CCGG C^CGG
1153 CCGG
NcoI 389 CCATGG C^CATGG
683 CCATGG
PflMI 1227 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACGCGATGG
SapI 1275 GCTCTTC GCTCTTC(1/4)
1293 GCTCTTC
TfiI 385 GAATC G^A[AT]TC
828 GAATC
Tru9I 299 TTAA T^TAA
449 TTAA
Van91I 1227 CCAGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACGCGATGG
XcmI 221 CCATCCTGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
770 CCAGTAGCTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI BbsI BbvI BmyI BpuAI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI