Restriction map for
pFN21AE6621
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII AccI AccIII AciI AclI
AcyI AflII AgeI AhdI AluI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BclI BcnI BfaI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
Ksp632I KspI MaeI MamI MfeI
MluNI MroI MscI MspA1I MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 101 GGTACC G^GTACC
AccII 136 CGCG CG^CG
AcsI 822 GAATTT [GA]^AATT[CT]
AlwNI 536 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 822 GAATTT [GA]^AATT[CT]
Asp718I 101 GGTACC G^GTACC
BbvCI 105 CCTCAGC CCTCAGC(-5/-2)
BciVI 684 GTATCC GTATCC(6/5)
BglI 277 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiYI 50 CCCTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 50 CCCTCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 906 GAGAC GTCTC(1/5)
BsmBI 906 GAGACG CGTCTC(1/5)
BspMI 960 GCAGGT ACCTGC(4/8)
BstAPI 960 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 249 GGTGACC G^GT[ACGT]ACC
BstPI 249 GGTGACC G^GT[ACGT]ACC
BstUI 136 CGCG CG^CG
BstXI 737 CCAGAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 282 GCAGGC GC[ACGT]^[ACGT]GC
DraI 582 TTTAAA TTT^AAA
DsaI 924 CCATGG C^C[GA][CT]GG
EcoO65I 249 GGTGACC G^GT[ACGT]ACC
HincII 899 GTCAAC GT[CT]^[GA]AC
HindII 899 GTCAAC GT[CT]^[GA]AC
HinfI 881 GAATC G^A[ACGT]TC
KpnI 101 GGTACC GGTAC^C
MluI 135 ACGCGT A^CGCGT
MvnI 136 CGCG CG^CG
NcoI 924 CCATGG C^CATGG
NspI 982 ACATGC [GA]CATG^[CT]
PstI 711 CTGCAG CTGCA^G
SnaBI 523 TACGTA TAC^GTA
TaqI 201 TCGA T^CGA
TfiI 881 GAATC G^A[AT]TC
TthHB8I 201 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 135 ACGCGT A^C[GA][CT]GT
909 ACGTGT
Asp700I 406 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
603 GAAGTTTTTC
BanI 101 GGTACC G^G[CT][GA]CC
747 GGTGCC
BbvI 919 GCAGC GCAGC(8/12)
69 GCTGC
BsaAI 523 TACGTA [CT]AC^GT[GA]
775 CACGTA
BsmI 207 GCATTC GAATGC(1/-1)
484 GCATTC
BsrI 143 ACTGG ACTGG(1/-1)
559 ACTGG
BssKI 278 CCAGG ^CC[ACGT]GG
678 CCAGG
BstNI 278 CCAGG CC^[AT]GG
678 CCAGG
EcoRII 278 CCAGG ^CC[AT]GG
678 CCAGG
Fnu4HI 69 GCTGC GC^[ACGT]GC
919 GCAGC
ItaI 69 GCTGC GC^[ACGT]GC
919 GCAGC
MseI 583 TTAA T^TAA
829 TTAA
MvaI 278 CCAGG CC^[AT]GG
678 CCAGG
MwoI 277 GCCAGGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
960 GCAGGTCATGC
ScrFI 278 CCAGG CC^[ACGT]GG
678 CCAGG
SfcI 642 CTATAG C^T[GA][CT]AG
711 CTGCAG
Tru9I 583 TTAA T^TAA
829 TTAA
TseI 69 GCTGC G^C[AT]GC
919 GCAGC
XmnI 406 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
603 GAAGTTTTTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AlwI BbsI BpuAI BsaJI
BstF5I BstYI DdeI DpnII EcoT14I
FokI HaeIII HphI MaeII MaeIII
MboI MboII MflI MnlI MslI
NdeII NlaIII NlaIV RsaI Sau3AI
StyI Tsp45I Tsp509I TspRI XhoII