Restriction map for
pFN21AE4014
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI ItaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TseI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 78 GTCTAC GT^[AC][GT]AC
AciI 25 CCGC CCGC(-3/-1)
AfaI 272 GTAC GT^AC
AlwNI 283 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 386 GAGCTC G[GA]GC[CT]^C
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BpmI 383 CTGGAG CTGGAG(16/14)
BsaWI 443 ACCGGA [AT]^CCGG[AT]
BseRI 545 GAGGAG GAGGAG(10/8)
BsmFI 57 GGGAC GGGAC(10/14)
Cfr13I 492 GGCCC G^G[ACGT]CC
DpnII 153 GATC ^GATC
EcoNI 127 CCTACAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeIII 492 GGCC GG^CC
HapII 444 CCGG C^CGG
HpaII 444 CCGG C^CGG
HphI 407 TCACC GGTGA(8/7)
MboI 153 GATC ^GATC
MslI 408 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MspI 444 CCGG C^CGG
MwoI 388 GCTCTGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 153 GATC ^GATC
NspI 377 ACATGC [GA]CATG^[CT]
PvuII 68 CAGCTG CAG^CTG
RsaI 272 GTAC GT^AC
SacI 386 GAGCTC GAGCT^C
SapI 525 GAAGAGC GCTCTTC(1/4)
Sau3AI 153 GATC ^GATC
Sau96I 492 GGCCC G^G[ACGT]CC
TaqI 155 TCGA T^CGA
TfiI 168 GAATC G^A[AT]TC
TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 155 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 313 GAGCAC G[AT]GC[AT]^C
386 GAGCTC
BsaJI 494 CCCAGG C^C[ACGT][ACGT]GG
495 CCAGGG
BsiHKAI 313 GAGCAC G[AT]GC[AT]^C
386 GAGCTC
BssKI 382 CCTGG ^CC[ACGT]GG
495 CCAGG
BstF5I 123 CATCC GGATG(2/0)
189 GGATG
BstNI 382 CCTGG CC^[AT]GG
495 CCAGG
Cac8I 102 GCTTGC GC[ACGT]^[ACGT]GC
489 GCTGGC
EarI 292 GAAGAG CTCTTC(1/4)
525 GAAGAG
EcoRII 382 CCTGG ^CC[AT]GG
495 CCAGG
FokI 123 CATCC GGATG(9/13)
189 GGATG
HindIII 100 AAGCTT A^AGCTT
430 AAGCTT
Ksp632I 292 GAAGAG CTCTTC(1/4)
525 GAAGAG
MvaI 382 CCTGG CC^[AT]GG
495 CCAGG
NlaIII 378 CATG CATG^
534 CATG
PleI 64 GACTC GAGTC(4/5)
295 GAGTC
ScrFI 382 CCTGG CC^[ACGT]GG
495 CCAGG
Tsp45I 346 GTGAC ^GT[GC]AC
440 GTGAC
Tsp509I 95 AATT ^AATT
509 AATT
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BmyI BsiYI BslI BsmAI
Bsp1286I BsrI DdeI HinfI MaeIII
MboII MnlI MseI Tru9I