Restriction map for
pFN21AE5245
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
TspRI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 328 GTAGAC GT^[AC][GT]AC
AfaI 531 GTAC GT^AC
AflIII 437 ACACGT A^C[GA][CT]GT
AluI 258 AGCT AG^CT
AlwI 413 GGATC GGATC(4/5)
AlwNI 142 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 214 ATTAAT AT^TAAT
AsnI 214 ATTAAT AT^TAAT
Asp700I 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 257 GAGCTC G[AT]GC[AT]^C
AvaII 63 GGACC G^G[AT]CC
AvrII 384 CCTAGG C^CTAGG
BanII 257 GAGCTC G[GA]GC[CT]^C
BbsI 165 GAAGAC GAAGAC(2/6)
BfaI 385 CTAG C^TAG
BlnI 384 CCTAGG C^CTAGG
BmyI 257 GAGCTC G[AGT]GC[ACT]^C
BpuAI 165 GAAGAC GAAGAC(2/6)
BsiHKAI 257 GAGCTC G[AT]GC[AT]^C
Bsp1286I 257 GAGCTC G[AGT]GC[ACT]^C
BspMI 65 ACCTGC ACCTGC(4/8)
BsrBI 79 CCGCTC CCGCTC(-3/-3)
BstXI 272 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 63 GGACC G^G[ACGT]CC
DpnII 414 GATC ^GATC
DraI 201 TTTAAA TTT^AAA
DraII 62 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 247 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 62 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 91 GAATTC G^AATTC
EcoT14I 384 CCTAGG C^C[AT][AT]GG
MaeI 385 CTAG C^TAG
MaeII 439 ACGT A^CGT
MboI 414 GATC ^GATC
MwoI 69 GCCCAGGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 414 GATC ^GATC
PflMI 36 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 62 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 214 ATTAAT AT^TAAT
RsaI 531 GTAC GT^AC
SacI 257 GAGCTC GAGCT^C
Sau3AI 414 GATC ^GATC
Sau96I 63 GGACC G^G[ACGT]CC
SexAI 169 ACCTGGT A^CC[AT]GGT
StyI 384 CCTAGG C^C[AT][AT]GG
TaqI 195 TCGA T^CGA
TthHB8I 195 TCGA T^CGA
Van91I 36 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 76 CCGC CCGC(-3/-1)
79 CCGC
AcsI 91 GAATTC [GA]^AATT[CT]
535 AAATTT
ApoI 91 GAATTC [GA]^AATT[CT]
535 AAATTT
BsiYI 36 CCAAAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCTCCACAAGG
BslI 36 CCAAAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCTCCACAAGG
BsmFI 62 GGGAC GGGAC(10/14)
33 GTCCC
BstF5I 282 GGATG GGATG(2/0)
555 GGATG
Fnu4HI 75 GCCGC GC^[ACGT]GC
78 GCCGC
FokI 282 GGATG GGATG(9/13)
555 GGATG
HaeIII 74 GGCC GG^CC
388 GGCC
HphI 150 GGTGA GGTGA(8/7)
381 TCACC
ItaI 75 GCCGC GC^[ACGT]GC
78 GCCGC
MaeIII 406 GTGAC ^GT[ACGT]AC
434 GTGAC
NlaIV 62 GGGACC GG[ACGT]^[ACGT]CC
243 GGAACC
Tsp45I 406 GTGAC ^GT[GC]AC
434 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BsaJI BssKI BstNI DdeI EcoRII
HinfI MboII MnlI MseI MvaI
NlaIII ScrFI SfaNI TfiI Tru9I
Tsp509I