Restriction map for
pFN21AE3021
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cac8I CelII
Cfr10I Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboII MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 67 CGCG CG^CG
AcyI 76 GACGCC G[GA]^CG[CT]C
AluI 199 AGCT AG^CT
AlwNI 144 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 86 TGATCA T^GATCA
BcnI 92 CCGGG CC^[GC]GG
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsaJI 92 CCGGGG C^C[ACGT][ACGT]GG
BsmAI 194 GAGAC GTCTC(1/5)
BsmFI 74 GGGAC GGGAC(10/14)
BsrI 189 ACTGG ACTGG(1/-1)
BstNI 268 CCAGG CC^[AT]GG
BstUI 67 CGCG CG^CG
BstXI 114 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 87 GATC ^GATC
EcoRII 268 CCAGG ^CC[AT]GG
EcoT22I 244 ATGCAT ATGCA^T
FauI 65 CCCGC CCCGC(4/6)
FbaI 86 TGATCA T^GATCA
HaeIII 276 GGCC GG^CC
HapII 92 CCGG C^CGG
HgaI 76 GACGC GACGC(5/10)
Hin1I 76 GACGCC G[GA]^CG[CT]C
HpaII 92 CCGG C^CGG
HphI 89 TCACC GGTGA(8/7)
MaeIII 101 GTGAC ^GT[ACGT]AC
MboI 87 GATC ^GATC
MseI 296 TTAA T^TAA
MslI 174 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 92 CCGG C^CGG
MvaI 268 CCAGG CC^[AT]GG
MvnI 67 CGCG CG^CG
NciI 92 CCGGG CC^[GC]GG
NdeII 87 GATC ^GATC
NlaIV 5 GGAGCC GG[ACGT]^[ACGT]CC
NsiI 244 ATGCAT ATGCA^T
NspI 128 GCATGT [GA]CATG^[CT]
PflMI 226 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 198 CAGCTG CAG^CTG
Sau3AI 87 GATC ^GATC
Tru9I 296 TTAA T^TAA
Tsp45I 101 GTGAC ^GT[GC]AC
TspRI 209 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 226 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BseRI 31 CTCCTC GAGGAG(10/8)
3 GAGGAG
BssKI 92 CCGGG ^CC[ACGT]GG
268 CCAGG
BstF5I 205 CATCC GGATG(2/0)
242 GGATG
CfoI 17 GCGC GCG^C
68 GCGC
FokI 205 CATCC GGATG(9/13)
242 GGATG
HhaI 17 GCGC GCG^C
68 GCGC
HinP1I 17 GCGC G^CGC
68 GCGC
HinfI 109 GACTC G^A[ACGT]TC
213 GACTC
MspA1I 49 CCGCTG C[AC]G^C[GT]G
198 CAGCTG
NlaIII 129 CATG CATG^
260 CATG
PleI 109 GACTC GAGTC(4/5)
213 GACTC
ScrFI 92 CCGGG CC^[ACGT]GG
268 CCAGG
SfaNI 243 GATGC GCATC(5/9)
246 GCATC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AciI BbvI BsiYI BslI Fnu4HI
ItaI MnlI MwoI TseI