Restriction map for
pFN21AE3068
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 359 ACATGT A^C[GA][CT]GT
Alw44I 416 GTGCAC G^TGCAC
AocI 558 CCTCAGG CC^T[ACGT]AGG
ApaI 234 GGGCCC GGGCC^C
ApaLI 416 GTGCAC G^TGCAC
Asp700I 477 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 416 GTGCAC G[AT]GC[AT]^C
AvaII 452 GGACC G^G[AT]CC
BalI 625 TGGCCA TGG^CCA
BanII 234 GGGCCC G[GA]GC[CT]^C
BclI 263 TGATCA T^GATCA
BfaI 385 CTAG C^TAG
BglI 108 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 416 GTGCAC G[AT]GC[AT]^C
BspHI 266 TCATGA T^CATGA
BspLU11I 359 ACATGT A^CATGT
BstBI 68 TTCGAA TT^CGAA
BstXI 224 CCAACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 140 AGATCC [GA]^GATC[CT]
Bsu36I 558 CCTCAGG CC^T[ACGT]AGG
DdeI 559 CTCAG C^T[ACGT]AG
DraI 202 TTTAAA TTT^AAA
DraII 234 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 382 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 529 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 326 CCACGG C^C[GA][CT]GG
EaeI 625 TGGCCA [CT]^GGCC[GA]
EarI 481 CTCTTC CTCTTC(1/4)
Eco81I 558 CCTCAGG CC^T[ACGT]AGG
EcoO109I 234 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 134 CCAAGG C^C[AT][AT]GG
FbaI 263 TGATCA T^GATCA
HinfI 270 GAGTC G^A[ACGT]TC
HphI 315 GGTGA GGTGA(8/7)
Ksp632I 481 CTCTTC CTCTTC(1/4)
MaeI 385 CTAG C^TAG
MaeII 227 ACGT A^CGT
MaeIII 49 GTCAC ^GT[ACGT]AC
MflI 140 AGATCC [GA]^GATC[CT]
MluNI 625 TGGCCA TGG^CCA
MscI 625 TGGCCA TGG^CCA
MslI 261 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 531 CAGCTG C[AC]G^C[GT]G
NspV 68 TTCGAA TT^CGAA
PleI 270 GAGTC GAGTC(4/5)
PstI 210 CTGCAG CTGCA^G
PvuII 531 CAGCTG CAG^CTG
RcaI 266 TCATGA T^CATGA
SfcI 210 CTGCAG C^T[GA][CT]AG
SfuI 68 TTCGAA TT^CGAA
SphI 653 GCATGC GCATG^C
StuI 297 AGGCCT AGG^CCT
StyI 134 CCAAGG C^C[AT][AT]GG
TaqI 69 TCGA T^CGA
Tsp45I 49 GTCAC ^GT[GC]AC
TthHB8I 69 TCGA T^CGA
XhoII 140 AGATCC [GA]^GATC[CT]
XmnI 477 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 221 CCGC CCGC(-3/-1)
258 CCGC
AcsI 391 AAATTC [GA]^AATT[CT]
404 AAATTC
AfaI 156 GTAC GT^AC
363 GTAC
AlwI 141 GATCC GGATC(4/5)
445 GATCC
ApoI 391 AAATTC [GA]^AATT[CT]
404 AAATTC
BbsI 253 GAAGAC GAAGAC(2/6)
538 GTCTTC
BcnI 409 CCGGG CC^[GC]GG
525 CCGGG
BmyI 234 GGGCCC G[AGT]GC[ACT]^C
416 GTGCAC
BpmI 368 CTGGAG CTGGAG(16/14)
637 CTGGAG
BpuAI 253 GAAGAC GAAGAC(2/6)
538 GTCTTC
Bsp1286I 234 GGGCCC G[AGT]GC[ACT]^C
416 GTGCAC
BsrI 420 ACTGG ACTGG(1/-1)
516 ACTGG
HapII 409 CCGG C^CGG
525 CCGG
HpaII 409 CCGG C^CGG
525 CCGG
MspI 409 CCGG C^CGG
525 CCGG
NciI 409 CCGGG CC^[GC]GG
525 CCGGG
NspI 359 ACATGT [GA]CATG^[CT]
653 GCATGC
RsaI 156 GTAC GT^AC
363 GTAC
Tsp509I 392 AATT ^AATT
405 AATT
TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^
419 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BsaJI BsmFI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI