Restriction map for
pFN21AE1653
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MfeI MflI MluI MslI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 416 TCCGGA T^CCGGA
AfaI 493 GTAC GT^AC
AspHI 46 GTGCTC G[AT]GC[AT]^C
AspI 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 354 GGGCTC G[GA]GC[CT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvCI 96 CCTCAGC CCTCAGC(-5/-2)
BbvI 54 GCTGC GCAGC(8/12)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaWI 416 TCCGGA [AT]^CCGG[AT]
BseAI 416 TCCGGA T^CCGGA
BsiHKAI 46 GTGCTC G[AT]GC[AT]^C
BsmAI 247 GAGAC GTCTC(1/5)
BspEI 416 TCCGGA T^CCGGA
BsrDI 84 CATTGC GCAATG(2/0)
BsrI 42 ACTGG ACTGG(1/-1)
BstXI 9 CCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 97 CTCAG C^T[ACGT]AG
DraI 448 TTTAAA TTT^AAA
DrdI 267 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 339 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 54 GCTGC GC^[ACGT]GC
HapII 417 CCGG C^CGG
HpaII 417 CCGG C^CGG
ItaI 54 GCTGC GC^[ACGT]GC
MroI 416 TCCGGA T^CCGGA
MseI 449 TTAA T^TAA
MspA1I 142 CAGCGG C[AC]G^C[GT]G
MspI 417 CCGG C^CGG
PflFI 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 21 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 505 GAGTC GAGTC(4/5)
RsaI 493 GTAC GT^AC
SfcI 125 CTATAG C^T[GA][CT]AG
Tru9I 449 TTAA T^TAA
TseI 54 GCTGC G^C[AT]GC
Tsp509I 456 AATT ^AATT
Tth111I 501 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 21 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 188 CCAGGCCCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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BalI 18 TGGCCA TGG^CCA
77 TGGCCA
BsmFI 206 GGGAC GGGAC(10/14)
328 GGGAC
DraII 92 AGGTCCT [GA]G^G[ACGT]CC[CT]
206 GGGACCC
EaeI 18 TGGCCA [CT]^GGCC[GA]
77 TGGCCA
EcoO109I 92 AGGTCCT [GA]G^G[ACGT]CC[CT]
206 GGGACCC
MluNI 18 TGGCCA TGG^CCA
77 TGGCCA
MscI 18 TGGCCA TGG^CCA
77 TGGCCA
PpuMI 92 AGGTCCT [GA]G^G[AT]CC[CT]
206 GGGACCC
TfiI 467 GAATC G^A[AT]TC
486 GAATC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AvaII BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I EcoRII FokI HaeIII
HinfI HphI MaeIII MboII MnlI
MvaI MwoI NlaIV Sau96I ScrFI
SfaNI Tsp45I TspRI