Restriction map for
pFN21AE1669
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeIII MfeI MflI MluI
MroI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AcsI 103 GAATTT [GA]^AATT[CT]
AfaI 512 GTAC GT^AC
AlwI 44 GGATC GGATC(4/5)
AlwNI 237 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 424 CCTCAGG CC^T[ACGT]AGG
ApoI 103 GAATTT [GA]^AATT[CT]
AvaI 119 CTCGGG C^[CT]CG[GA]G
AvaII 275 GGTCC G^G[AT]CC
AviII 329 TGCGCA TGC^GCA
BalI 50 TGGCCA TGG^CCA
BbsI 186 GTCTTC GAAGAC(2/6)
BfaI 529 CTAG C^TAG
BpmI 488 CTCCAG CTGGAG(16/14)
BpuAI 186 GTCTTC GAAGAC(2/6)
BsaAI 509 CACGTA [CT]AC^GT[GA]
BsaBI 39 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 163 GAGGAG GAGGAG(10/8)
BsgI 506 CTGCAC GTGCAG(16/14)
BsoBI 119 CTCGGG C^[CT]CG[GA]G
BsrI 72 ACTGG ACTGG(1/-1)
BssSI 472 CTCGTG CACGAG(-5/-1)
BstAPI 321 GCACGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 462 GGATG GGATG(2/0)
Bsu36I 424 CCTCAGG CC^T[ACGT]AGG
CfoI 330 GCGC GCG^C
DdeI 425 CTCAG C^T[ACGT]AG
DpnII 45 GATC ^GATC
DraII 274 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 206 CCATGG C^C[GA][CT]GG
EaeI 50 TGGCCA [CT]^GGCC[GA]
Eco81I 424 CCTCAGG CC^T[ACGT]AGG
EcoO109I 274 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 206 CCATGG C^C[AT][AT]GG
FauI 20 CCCGC CCCGC(4/6)
FokI 462 GGATG GGATG(9/13)
FspI 329 TGCGCA TGC^GCA
HhaI 330 GCGC GCG^C
HinP1I 330 GCGC G^CGC
HinfI 486 GACTC G^A[ACGT]TC
MaeI 529 CTAG C^TAG
MaeII 510 ACGT A^CGT
MamI 39 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 45 GATC ^GATC
MluNI 50 TGGCCA TGG^CCA
MscI 50 TGGCCA TGG^CCA
MslI 519 CACTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 414 CCGCTG C[AC]G^C[GT]G
NcoI 206 CCATGG C^CATGG
NdeII 45 GATC ^GATC
NspI 266 GCATGC [GA]CATG^[CT]
PleI 486 GACTC GAGTC(4/5)
PpuMI 274 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 6 CTGCAG CTGCA^G
RsaI 512 GTAC GT^AC
Sau3AI 45 GATC ^GATC
SfaNI 536 GCATC GCATC(5/9)
SfcI 6 CTGCAG C^T[GA][CT]AG
SphI 266 GCATGC GCATG^C
StyI 206 CCATGG C^C[AT][AT]GG
TspRI 33 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 12 enzymes) #
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AspHI 319 GAGCAC G[AT]GC[AT]^C
466 GAGCAC
BciVI 124 GGATAC GTATCC(6/5)
212 GGATAC
BcnI 86 CCGGG CC^[GC]GG
439 CCCGG
BsiHKAI 319 GAGCAC G[AT]GC[AT]^C
466 GAGCAC
BspMI 241 ACCTGC ACCTGC(4/8)
262 ACCTGC
DraIII 322 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
469 CACCTCGTG
HapII 86 CCGG C^CGG
440 CCGG
HpaII 86 CCGG C^CGG
440 CCGG
MboII 12 GAAGA GAAGA(8/7)
187 TCTTC
MspI 86 CCGG C^CGG
440 CCGG
NciI 86 CCGGG CC^[GC]GG
439 CCCGG
Tsp509I 104 AATT ^AATT
174 AATT
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I EcoRII Fnu4HI HaeIII
ItaI MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI