Restriction map for
pFN21AE3230
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccII AclI AflII AflIII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BfrI BlnI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1163 GACGTC GACGT^C
AccI 1534 GTCTAC GT^[AC][GT]AC
AccIII 1602 TCCGGA T^CCGGA
AcyI 1163 GACGTC G[GA]^CG[CT]C
AlwI 1035 GATCC GGATC(4/5)
Aor51HI 791 AGCGCT AGC^GCT
Asp700I 87 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 91 GTCTTC GAAGAC(2/6)
BcgI 9 GCACGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1051 GTATCC GTATCC(6/5)
BglII 123 AGATCT A^GATCT
BlpI 732 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 732 GCTTAGC GC^T[ACGT]AGC
BpuAI 91 GTCTTC GAAGAC(2/6)
BsaHI 1163 GACGTC G[GA]^CG[CT]C
BsaWI 1602 TCCGGA [AT]^CCGG[AT]
BseAI 1602 TCCGGA T^CCGGA
BsmI 1201 GCATTC GAATGC(1/-1)
BspEI 1602 TCCGGA T^CCGGA
BspHI 1022 TCATGA T^CATGA
BsrDI 882 CATTGC GCAATG(2/0)
BstXI 1091 CCACAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 732 GCTTAGC GC^T[ACGT]AGC
CfoI 792 GCGC GCG^C
DraI 1006 TTTAAA TTT^AAA
DrdI 880 GACATTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 791 AGCGCT AGC^GCT
EcoRV 217 GATATC GAT^ATC
FauI 283 GCGGG CCCGC(4/6)
HaeII 791 AGCGCT [GA]GCGC^[CT]
HgaI 847 GCGTC GACGC(5/10)
HhaI 792 GCGC GCG^C
Hin1I 1163 GACGTC G[GA]^CG[CT]C
HinP1I 792 GCGC G^CGC
HincII 973 GTTGAC GT[CT]^[GA]AC
HindII 973 GTTGAC GT[CT]^[GA]AC
MroI 1602 TCCGGA T^CCGGA
PaeR7I 1567 CTCGAG C^TCGAG
PpuMI 490 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1084 CTGCAG CTGCA^G
RcaI 1022 TCATGA T^CATGA
ScaI 192 AGTACT AGT^ACT
XhoI 1567 CTCGAG C^TCGAG
XmnI 87 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AfaI 193 GTAC GT^AC
745 GTAC
Alw44I 160 GTGCAC G^TGCAC
710 GTGCAC
ApaLI 160 GTGCAC G^TGCAC
710 GTGCAC
BalI 1250 TGGCCA TGG^CCA
1310 TGGCCA
BanI 703 GGTGCC G^G[CT][GA]CC
898 GGCACC
BanII 320 GAGCCC G[GA]GC[CT]^C
931 GAGCCC
BfaI 268 CTAG C^TAG
1501 CTAG
BglI 322 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1396 GCCAACTCGGC
BseRI 724 GAGGAG GAGGAG(10/8)
1319 GAGGAG
BsmAI 864 GAGAC GTCTC(1/5)
1584 GTCTC
BspMI 54 GCAGGT ACCTGC(4/8)
1444 GCAGGT
BstYI 123 AGATCT [GA]^GATC[CT]
1034 AGATCC
EaeI 1250 TGGCCA [CT]^GGCC[GA]
1310 TGGCCA
EcoT14I 612 CCTTGG C^C[AT][AT]GG
699 CCTTGG
HindIII 339 AAGCTT A^AGCTT
730 AAGCTT
MaeI 268 CTAG C^TAG
1501 CTAG
MaeII 785 ACGT A^CGT
1164 ACGT
MflI 123 AGATCT [GA]^GATC[CT]
1034 AGATCC
MluNI 1250 TGGCCA TGG^CCA
1310 TGGCCA
MscI 1250 TGGCCA TGG^CCA
1310 TGGCCA
MseI 1007 TTAA T^TAA
1175 TTAA
PflMI 130 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCAGGCACTGG
PvuII 442 CAGCTG CAG^CTG
477 CAGCTG
RsaI 193 GTAC GT^AC
745 GTAC
SfcI 636 CTACAG C^T[GA][CT]AG
1084 CTGCAG
SmaI 1518 CCCGGG CCC^GGG
1592 CCCGGG
SmlI 260 CTCAAG C^T[CT][GA]AG
1567 CTCGAG
StuI 554 AGGCCT AGG^CCT
1272 AGGCCT
StyI 612 CCTTGG C^C[AT][AT]GG
699 CCTTGG
TfiI 1260 GATTC G^A[AT]TC
1577 GAATC
Tru9I 1007 TTAA T^TAA
1175 TTAA
Van91I 130 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCAGGCACTGG
XhoII 123 AGATCT [GA]^GATC[CT]
1034 AGATCC
XmaCI 1518 CCCGGG C^CCGGG
1592 CCCGGG
XmaI 1518 CCCGGG C^CCGGG
1592 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwNI ApoI
AspHI AvaI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI